Gene expression profiling of IL4I1 KO and WT CD8+ T-cell subsets from TCL1-AT mice
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA630264 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Analysis of the effect of IL4I1 on gene expression of CD8 T-cells in CLL
2020-08-19 | GSE149846 | GEO
Project description:We studied the role of the histone methyltransferase DOT1L in T cell development and differentiation. Here we generated RNA-seq data from sorted CD8 subpopulations from WT (Lcre+/-) and Dot1L KO (Lcre+/-;Dot1Lfl/fl) mice. This data was to define tissue specific expression signatures and it was compared with H3K79me2 ChIP-seq data from the same subsets.
2020-07-29 | GSE138910 | GEO
Project description:RNA expression in WT and Dot1L KO CD8 T cell subsets
| PRJNA577714 | ENA
Project description:Tcl1 is known to be involved in survival, proliferation and differentiation of human lymphocytes and mouse embryonic stem cells. Loss of Tcl1 gene in the KO mouse model affects skin integrity inducing alopecia and ulcerations. The study used epidermal keratinocytes from wild type (WT), Tcl1 knock down (KO) and K14-driven TCL1 transgenic (TGKO) mouse models to investigate the role of Tcl1 gene in the skin homeostasis. Our data indicate that Tcl1 loss mainly induces a decrease in the gene expression of keratinocytes, which is largely rescued by K14-TCL1. GO analysis revealed Tcl1 to be involved in the proliferation and differentiation of mouse keratinocytes. Moreover, pathway analysis identifies growth factor and MAPK signaling to be highly implicated.
2018-08-10 | GSE118345 | GEO
Project description:Tcl1 tg mice develop a chronic lymphocytic leukemia (CLL) -like disease. To investigate the contribution of the adhesion molecule CD44 to CLL pathophysiology, we developed a CD19Cre CD44flox/flox Tcl1 tg mouse with a B cell specific CD44 deficiency (CD44ΔB Tcl1 tg). We used the Clariom S mouse microarray from Affymetrix to investigate transcriptional differeneces between Tcl1 tg and CD44ΔB Tcl1 tg mice
2018-01-13 | GSE109121 | GEO
Project description:Human Naïve-like CD8 T cells induced by the Yellow Fever Vaccine 17D were compared to the conventional subsets in total CD8 T cells Samples originate from peripheral blood mononuclear cells (PBMC) from 8 different donors vaccinated with the YF-17D vaccine 1'000 cells from various CD8 T cells subsets were purified by flow cytometry, from 8 vaccinees (donors d1 to d8); the subsets (cell types) include: A2/NS4b tetramer positive CCR7+ CD45RA+ CD8 T cells (A2_NS4b Naïve-like), Total Naive (CCR7+ CD45RA+), Total Tscm (CCR7+ CD45RA+ CD58+ CD95+), Total CM (CCR7+ CD45RA-) and Total Effectors (CCR7 negative).
2015-04-08 | E-GEOD-65804 | biostudies-arrayexpress
Project description:Tcl1 is one of embryonic stem cell specific transcripts identified by digital differential display analysis. Tcl1 is highly expressed during early embryogenesis and to some extent in embryonic stem cells. Its expression in adult tissues is restricted to testis and ovary, and is found to decline if Oct-3/4 expression is suppressed in embryonic stem cells. It is reported that the female knockout mice exhibit reduced fertility. It was first identified in human T-cell tumors and the tumorigenic property was proved by a series of transgenic studies. We have used DNA microarray to identify potential Tcl1 targets in embryonic stem cells and the influence of Tcl1 on stemness. Experiment Overall Design: We analyzed two arrays, each comparing expression profiles of a Tcl1 gene disruptant and a Tcl1 stable transformant. We used different clones with their dyes swapped.(biological replication)
2008-06-13 | E-GEOD-4801 | biostudies-arrayexpress
Project description:The aim was to assess miRNA expression in 3 human ex-vivo CD8+ T cell subsets which span from antigen inexperienced cells (NaM-CM-/ve) to early memory cells (central memory, Tcm) and later stage memory cells (effector memory, Tem) CD8+ T cells were sorted on a FACS Aria II machine. N = naM-CM-/ve = CD8+, CCR7+, CD45RA+, CD45RO-, Tcm = central memory = CD8+, CCR7+, CD45RA-, CD45RO-,Tem= effector memory = CD8+, CCR7-, CD45RA-, CD45RO+ PBMC were isolated from 3 healthy human donors and sorted by FACS into 3 CD8+ T cell subsets. Total RNA was purified using the miRVANA kit (Ambion)
2014-10-24 | E-GEOD-54867 | biostudies-arrayexpress
Project description:Tcl1 is one of embryonic stem cell specific transcripts identified by digital differential display analysis. Tcl1 is highly expressed during early embryogenesis and to some extent in embryonic stem cells. Its expression in adult tissues is restricted to testis and ovary, and is found to decline if Oct-3/4 expression is suppressed in embryonic stem cells. It is reported that the female knockout mice exhibit reduced fertility. It was first identified in human T-cell tumors and the tumorigenic property was proved by a series of transgenic studies. We have used DNA microarray to identify potential Tcl1 targets in embryonic stem cells and the influence of Tcl1 on stemness. Keywords: genetic modification
2007-05-10 | GSE4801 | GEO
Project description:Effector CD8+ T cells are believed to be terminally differentiated cells having cytotoxic activity and the ability to produce effector cytokines such as INF-M-NM-3 and TNF-M-NM-1. We investigated the difference between CXCR1+ and CXCR1- subsets of human effector CD27-CD28-CD8+ T cells. Both subsets similarly expressed cytolytic molecules and exerted substantial cytolytic activity, whereas only the CXCR1- subset had IL-2 productivity and self-proliferative activity and was more resistant to cell death than the CXCR1+ subset. These differences were explained by the specific up-regulation of CAMK4, SPRY2, and IL-7R in the CXCR1- subset and that of pro-apoptotic DAPK1 in the CXCR1+ subset. The IL-2 producers were more frequently found in the IL-7R+ subset of the CXCR1- effector CD8+ T cells than in the IL-7R- subset. IL-7/IL-7R signaling promoted cell survival only in the CXCR1- subset. The present study has highlighted a novel subset of effector CD8+ T cells producing IL-2 and suggests the importance of this subset in the homeostasis of effector CD8+ T cells. To find the genes that potentially cause the difference in IL-2 productivity and cell survival between the CXCR1+ and CXCR1- human effector CD8+ T cell subsets, we performed microarray gene expression analysis. We highly purified each CD3+CD8+ subset for this analysis (> 95.3%) from 5 healthy individuals, and then compared the gene expression profiles of each subset.
2011-01-27 | E-GEOD-26890 | biostudies-arrayexpress