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Inhibitor of Differentiation 4 (ID4) represses myoepithelial differentiation of mammary stem cells through its interaction with HEB

ABSTRACT: Inhibitor of Differentiation 4 (ID4) represses myoepithelial differentiation of mammary stem cells through its interaction with HEB

PROVIDER: PRJNA630706 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11712300_1.fastq.gz	Fastqsanger.gz
	SRR11712300_2.fastq.gz	Fastqsanger.gz
	SRR11712400_1.fastq.gz	Fastqsanger.gz
	SRR11712400_2.fastq.gz	Fastqsanger.gz
	SRR11712301_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Inhibitor of Differentiation 4 (ID4) represses myoepithelial differentiation of mammary stem cells through its interaction with HEB

Project description:Differentiation of stem cells embedded within the mammary epithelium is orchestrated by lineage-specifying transcription factors. Unlike the well-defined luminal hierarchy, dissection of the basal lineage has been hindered by a lack of specific markers. Inhibitor of Differentiation 4 (ID4) is a basally-restricted helix-loop-helix (HLH) transcription factor essential for mammary development. Here we show that ID4 is highly expressed in basal stem cells and decreases during myoepithelial differentiation. By integrating transcriptomic, proteomic and chromatin-association data we reveal that ID4 is required to suppress myoepithelial gene expression and cell fate.

2021-01-25 | PXD017517 | Pride

HEB ChIP-seq in ID4 knockdown Comma-Dβ mammary epithelial cells

Project description:We performed ChIP-seq for the E-protein transcription factor HEB in ID4 knockdown mammary epithelial cells to determine how ID4 affects HEB DNA binding.

2020-05-07 | GSE149968 | GEO

Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.

Project description:Inhibitor of differentiation (ID) proteins dimerize with basic HLH (bHLH) transcription factors, repressing transcription of lineage-specification genes across diverse cellular lineages. ID4 is a key regulator of mammary stem cells; however, the mechanism by which it achieves this is unclear. Here, we show that ID4 has a cell autonomous role in preventing myoepithelial differentiation of basal cells in mammary organoids and in vivo. ID4 positively regulates proliferative genes and negatively regulates genes involved in myoepithelial function. Mass spectrometry reveals that ID4 interacts with the bHLH protein HEB, which binds to E-box motifs in regulatory elements of basal developmental genes involved in extracellular matrix and the contractile cytoskeleton. We conclude that high ID4 expression in mammary basal stem cells antagonizes HEB transcriptional activity, preventing myoepithelial differentiation and allowing for appropriate tissue morphogenesis. Downregulation of ID4 during pregnancy modulates gene regulated by HEB, promoting specialization of basal cells into myoepithelial cells.

| S-EPMC7851187 | biostudies-literature

HEB ChIP-seq in ID4 knockdown Comma-Dβ mammary epithelial cells

Project description:HEB ChIP-seq in ID4 knockdown Comma-Dβ mammary epithelial cells

| PRJNA630713 | ENA

Inhibitor of Differentiation 4 (ID4) represses myoepithelial differentiation of mammary stem cells through its interaction with HEB

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-05-07 | GSE149969 | GEO

RNA-seq of ID4 knockdown Comma-Dβ mammary epithelial cells

Project description:The goal of this study was to determine the genes regulated by ID4 in mammary epithelial cells. ID4 was knocked down by siRNA in the mammary epithelial cell line Comma-Dβ and RNA-seq was performed following 48 hr to determine differentially expressed genes.

2020-05-07 | GSE149966 | GEO

RNA sequencing of ID4-GFP Bright and ID4-GFP Dim spermatogonia from postnatal day 8 transgenic Id4-Gfp (LT11) mouse pups and ID4-GFP+ spermatogonia from ID4 overexpression mouse model and control

Project description:Mice that constitutively overexpress ID4 in germ cells have impaired spermatogenic lineage development. The transcriptome of ID4-GFP+ spermatogonia from testes of ID4 overexpression animals was compared to the ID4-GFP+ population from controls

2017-01-20 | GSE93866 | GEO

Expression data from CD19-positive splenic B cells isolated from 1-month old ID4+/-TCL1-tg and ID4+/+TCL1-tg mice

Project description:The function of ID4 in CLL development was studied in vivo using TCL1 transgenic mouse model that develop leukemia similar to human CLL. TCL1 mice with ID4 single knockout gene have accelerated CLL progression. Results from the animal study suggest ID4 as a tumor suppressor gene that might regulate cell proliferation and apoptosis in B lymphocytes. Gene expression in CD19-positive splenic B cells collected from 1-month old ID4+/-TCL1-tg and ID4+/+TCL1-tg mice was compared by microarray, the goal is to find ID4-regulated genes involved in CLL development.

2010-11-04 | E-GEOD-25100 | biostudies-arrayexpress

Proteomic and phosphoproteomic profiling of aging human mammary myoepithelial and luminal epithelial cells

Project description:The aim of this project is to examine age-dependent changes in the proteomes and phosphoproteomes of normal human mammary luminal epithelial and myoepithelial cells.

2021-11-03 | PXD027540 | Pride

Brown adipocytes can display a mammary basal myoepithelial cell phenotype in vivo

Project description:A comparison of the mRNAs analysis in lactation mammary myoepithelial cells (GFP+/GFP-) and brown adipocytes (GFP+) from Myf5-Cre-ROSAmTmG and Ucp1-iCre-ROSAmTmG mice. Results provide the gene expression signature in the brown origin (UCP1/Myf5-positive) myoepithelial cells in vivo.

2017-01-07 | GSE93230 | GEO

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