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Acinetobacter lactucae strain:QL-1 | isolate:QL-1

ABSTRACT: Acinetobacter lactucae strain:QL-1 | isolate:QL-1 Genome sequencing

PROVIDER: PRJNA630840 | ENA |

REPOSITORIES: ENA

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Project description:Quorum sensing (QS) is a cell-cell communication mechanism among bacterial populations that is regulated through gene expression in response to cell density. The pathogenicity of Xanthomonas campestris pv. campestris (Xcc) is modulated by the diffusible signal factor (DSF)-mediated QS system. DSF is widely conserved in a variety of gram-negative bacterial pathogens. In this study, DSF-degrading bacteria and their enzymes were thoroughly explored as a biocontrol agent against Xcc. The results indicated that a novel DSF-degrading bacterium, Acinetobacter lactucae QL-1, effectively attenuated Xcc virulence through quorum quenching. Lab-based experiments indicated that plants inoculated with QL-1 and Xcc had less tissue decay than those inoculated with Xcc alone. Co-inoculation of strains Xcc and QL-1 significantly reduced the incidence and severity of disease in plants. Similarly, the application of crude enzymes of strain QL-1 substantially reduced the disease severity caused by Xcc. The results showed that strain QL-1 and its enzymes possess promising potential, which could be further investigated to better protect plants from DSF-dependent pathogens.

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Whole-Genome Sequencing Analysis of Quorum Quenching Bacterial Strain Acinetobacter lactucae QL-1 Identifies the FadY Enzyme for Degradation of the Diffusible Signal Factor.

Project description:The diffusible signal factor (DSF) is a fatty acid signal molecule and is widely conserved in various Gram-negative bacteria. DSF is involved in the regulation of pathogenic virulence in many bacterial pathogens, including Xanthomonas campestris pv. campestris (Xcc). Quorum quenching (QQ) is a potential approach for preventing and controlling DSF-mediated bacterial infections by the degradation of the DSF signal. Acinetobacter lactucae strain QL-1 possesses a superb DSF degradation ability and effectively attenuates Xcc virulence through QQ. However, the QQ mechanisms in strain QL-1 are still unknown. In the present study, whole-genome sequencing and comparative genomics analysis were conducted to identify the molecular mechanisms of QQ in strain QL-1. We found that the fadY gene of QL-1 is an ortholog of XccrpfB, a known DSF degradation gene, suggesting that strain QL-1 is capable of inactivating DSF by QQ enzymes. The results of site-directed mutagenesis indicated that fadY is required for strain QL-1 to degrade DSF. The determination of FadY activity in vitro revealed that the fatty acyl-CoA synthetase FadY had remarkable catalytic activity. Furthermore, the expression of fadY in transformed Xcc strain XC1 was investigated and shown to significantly attenuate bacterial pathogenicity on host plants, such as Chinese cabbage and radish. This is the first report demonstrating a DSF degradation enzyme from A. lactucae. Taken together, these findings shed light on the QQ mechanisms of A. lactucae strain QL-1, and provide useful enzymes and related genes for the biocontrol of infectious diseases caused by DSF-dependent bacterial pathogens.

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