Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome profiling of Clostridium tetani

ABSTRACT: Transcriptome profiling of Clostridium tetani

PROVIDER: PRJNA631247 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11735800.fastq.gz	Fastqsanger.gz
	SRR11735801.fastq.gz	Fastqsanger.gz
	SRR11735802.fastq.gz	Fastqsanger.gz
	SRR11735803.fastq.gz	Fastqsanger.gz
	SRR11735796.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Transcriptome profiling of Clostridium tetani

Project description:Clostridium tetani produces the tetanus-causing tetanus toxin (TeNT), one of the most powerful bacterial toxins known to humankind. The regulation of toxin expression is complex and involves the alternative sigma factor TetR as well as other regulators. Here, we identified a novel regulatory molecule, a non-coding small regulatory RNA (sRNA), located in the 3’ untranslated region of the tent gene. We show with an antisense RNA approach and recombinant expression of the tent locus with and without the sRNA in C. tetani strains that the sRNA acts as a negative regulator of TeNT expression and modulates the growth pattern of C. tetani. Its possible role is to limit tetanus toxin levels in the exponential growth phase; thus, it might interlock bacterial growth and toxin production.

2020-07-31 | GSE150141 | GEO

Clostridium tetani biomarkers for culture condition

Project description:We used RNA-seq to determine Clostridium tetani gene expression changes in response to culture conditions and time. Changes in response to time were more pronounced than those in response to culture conditions. The tetanus toxin gene is always highly expressed but does show expression changes between culture conditions. These results may become part of an approach to reduce animal testing during vaccine manufacturing.

2022-01-02 | GSE182408 | GEO

Clostridium tetani

Project description:Genome project of Clostridium tetani

| PRJEB437 | ENA

Clostridium tetani

Project description:Clostridium tetani Genome sequencing and assembly

| PRJNA265068 | ENA

Clostridium tetani

Project description:Clostridium tetani - Harvard 49205 Genome sequencing

| PRJNA514962 | ENA

Clostridium tetani

Project description:Genome of vaccine strains: Clostridium Tetani

| PRJNA341856 | ENA

Determining genetic stability in Clostridium tetani vaccine strains

Project description:To develop a method for improved assessment of the genetic stability of bacterial vaccine strains, we applied next-generation sequencing to a Clostridium tetani model strain (including inter- and intra-lab replicates) and other strains. Data were processed to determine (gain or loss of) gene copy numbers. Strains could easily be distiguished based on gain/loss of prophage-like and CRISPR/Cas genes. We found that the model strain has multiple copies of the plasmid carrying the gene coding for tetanus toxin as well as several other genes. Data were reproducible within and between laboratories. The limit of detection of our method is an order of magnitude better than that of the pulsed-field gel electrophoresis (PFGE) currently used during manufacturing. This approach may part of an approach to reduce animal testing during vaccine manufacturing.

2020-02-15 | GSE144242 | GEO

Clostridium tetani

Project description:Whole genome sequencing of Clostridium tetani, an opportunistic pathogen

| PRJDB2458 | ENA

Clostridium tetani

Project description:Whole genome analysis of Clostridium tetani strains isolated in Japan

| PRJDB14332 | ENA

Clostridium tetani strain:mfbjulcb2

Project description:Clostridium tetani strain:mfbjulcb2 Genome sequencing

| PRJNA431690 | ENA