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Effect of loss of Ecdysone-induced protein 93F (E93) on the Drosophila melanogaster antennal transcriptome.

ABSTRACT: Effect of loss of Ecdysone-induced protein 93F (E93) on the Drosophila melanogaster antennal transcriptome.

PROVIDER: PRJNA631739 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11772142_1.fastq.gz	Fastqsanger.gz
	SRR11772142_2.fastq.gz	Fastqsanger.gz
	SRR11772143_1.fastq.gz	Fastqsanger.gz
	SRR11772143_2.fastq.gz	Fastqsanger.gz
	SRR11772144_1.fastq.gz	Fastqsanger.gz

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Effect of loss of Ecdysone-induced protein 93F (E93) on the Drosophila melanogaster antennal transcriptome.

Project description:Ecdysone-induced protein 93F (E93) regulates local chromatin structure and/or gene transcription, raising the question of whether expression of Ir75c, Ir75b and Ir75a receptors in antenna is affected at the transcriptional level in the absence of this protein. We performed bulk RNA-sequencing (RNA-seq) of control and E93RNAi antennae. In control RNA-seq samples, we predicted the existence of seven distinct transcripts encoded by these three receptor genes. Notably, many transcripts initiating from Ir75c do not terminate at the 3’ end of this gene but rather run through the Ir75b and Ir75a exons. Similarly, a majority of transcripts initiating from Ir75b incorporate exons 2-7 of Ir75a. The pattern of transcription is consistent with the absence of canonical transcription termination/polyadenylation sequences downstream of the Ir75c and Ir75b coding regions; such a sequence is only present 3’ of Ir75a. All transcripts initiating from Ir75c are highly up-regulated in the absence of E93, consistent with the ectopic expression of receptor protein. Transcription initiating from Ir75b is strongly diminished, concordant with the loss of Ir75b protein expression. By contrast, Ir75a transcripts levels were not significantly lower in E93RNAi antennae, despite the loss of detectable Ir75a protein.

2021-01-31 | GSE150296 | GEO

Drosophila E93 promotes adult development and suppresses larval responses to ecdysone during metamorphosis

Project description:Pulses of the steroid hormone ecdysone act through transcriptional cascades to direct the major developmental transitions during the Drosophila life cycle. These include the prepupal ecdysone pulse, which occurs 10 hours after pupariation and triggers the onset of adult morphogenesis and larval tissue destruction. E93 encodes a transcription factor that is specifically induced by the prepupal pulse of ecdysone, supporting a model proposed by earlier work that it specifies the onset of adult development. Although a number of studies have addressed these functions for E93, little is known about its roles in the salivary gland where the E93 locus was originally identified. Here we show that E93 is required for development through late pupal stages, with mutants displaying defects in adult differentiation and no detectable effect on the destruction of salivary glands. RNA-seq analysis demonstrates that E93 regulates genes involved in development and morphogenesis in the salivary glands, but has little effect on cell death gene expression. We also show that E93 is required to direct the proper timing of ecdysone-regulated gene expression in salivary glands, and that it suppresses earlier transcriptional programs that occur during larval and prepupal stages. These studies support the model that the stage-specific induction of E93 in late prepupae provides a critical signal that defines the end of larval development and the onset of adult differentiation.

2022-08-11 | GSE181948 | GEO

Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster.

Project description:Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster.

2015-01-23 | PXD001455 | Pride

D. melanogaster eye-antennal imaginal discs developmental transcriptome

Project description:Transcriptomes of Drosophila melanogaster eye-antennal imaginal discs at three sequential larval stages: late 2nd instar (72h after egg laying (AEL)), mid 3rd instar (96h AEL) and late 3rd instar (120h AEL).

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Comparative transcriptomic analysis of the Drosophila melanogaster antennal olfactory subsystems.

Project description:We identified genes whose expression is enriched in the D. melanogaster antennal olfactory subsystems that express Odorant receptors (Ors) or Ionotropic receptors (Irs) by microarray analysis of RNA extracted from antennae of wild-type (Oregon R P2) animals, ato mutants (which lack the Ir subsystem) and amos mutants (which lack the Or subsystem).

2021-09-12 | GSE183763 | GEO

Identification of E93 binding sites in Drosophila melanogaster

Project description:Identification of E93 binding sites in Drosophila melanogaster

| PRJEB11893 | ENA

Drosophila E93 promotes adult development and suppresses larval responses to ecdysone during metamorphosis

Project description:Drosophila E93 promotes adult development and suppresses larval responses to ecdysone during metamorphosis

| PRJNA754032 | ENA

Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown

Project description:This SuperSeries is composed of the following subset Series: GSE30686: Gene expression analysis of Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown GSE30740: Distribution of Drosophila insulator proteins after ecdysone treatment in Kc cells Refer to individual Series

2011-10-13 | E-GEOD-30741 | biostudies-arrayexpress

Transcriptomic analysis of wild-type and amos mutant D. melanogaster third-instar larval antennal discs and adult antennae

Project description:Comparative transcriptomic analysis of Drosophila melanogaster third-instar larval antennal discs and adult antennae from wild-type animals and amos mutant animals.

2021-12-13 | GSE190696 | GEO

Gene expression analysis of Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown

Project description:This study examines the changes in genes expression that occur in Drosophila melanogaster during the ecdysone response as well as during RNAi knockdown of the insulator protein, CP190. Analysis was performed in Kc cells after 0, 3, and 48 hours of ecdysone treatment in the presence of either control or CP190 knockdown.

2011-10-14 | GSE30686 | GEO

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