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Effects of 4-nitrophenol on testicular somatic cells [TM4]

ABSTRACT: Effects of 4-nitrophenol on testicular somatic cells [TM4]

PROVIDER: PRJNA632419 | ENA |

REPOSITORIES: ENA

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Effects of 4-nitrophenol on testicular somatic cells

Project description:Effects of 4-nitrophenol on testicular somatic cells

| PRJNA632416 | ENA

Effects of 4-nitrophenol on testicular somatic cells [TM3]

Project description:Effects of 4-nitrophenol on testicular somatic cells [TM3]

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Project description:These data suggested that TM3 and TM4 cells showed different responses to PNP, which might mediate different toxic mechanisms.

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RNA-seq of TM3 Leydig and TM4 Sertoli cells under MEHP exposure

Project description:This study was designed to investigate the toxicological effects of MEHP on TM3 Leydig and TM4 Sertoli cells.

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Genome-wide analysis of gene expression in TM4 Sertoli cells following siRNA-mediated Gata4 inhibition

Project description:The effects of Gata4 inhibition on global gene expression in TM4 Sertoli cells was analyzed. Using microarray analysis we identified a set of genes that are downregulated or upregulated following siRNA-mediated silencing of Gata4 in the murine Sertoli cell line TM4. Among the downregulated genes were regulators of blood-testtis barrier function and lactate metabolism. TM4 cells were transfected with non-targeting (NT) siRNA and Gata4 siRNA respectively. 72h post transfection cells were lysed and RNA was extracted.

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Vitamin C modulates function and gene expression of mouse testis TM4 Sertoli cells

Project description:Vitamin C (ascorbic acid, AA), as essential micro-nutrient and antioxidant, can affect multiple cellular processes. Previously, we showed that Vitamin C supplement could promote the proliferation and suppress apoptosis of porcine Sertoli cells, via modulating the global nucleic acid methylation levels to alter transcriptome and proteomics. Due to the differences between pig and rodent species, here we investigated the effects of Vitamin C on mouse TM4 Sertoli cells. Vitamin C (250µM) treatment of mouse TM4 Sertoli cells for 36h could significantly increase cell viability, promote cell proliferation and decrease the cell percentage with early apoptosis. Vitamin C (250µM, 36h) also significantly decreased ROS level, elevated mitochondrial function, altered the levels of nucleic acid N6-methylation (m6A) and modified the levels of histone H3 trimethylation (H3K4me3, H3K9Me3 and H3K36me3) of mouse TM4 Sertoli cells. Vitamin C (250µM, 36h) promoted the secretion of anti-müllerian hormone and Estrodiol, but decreased lactate metabolite in mouse TM4 Sertoli cells. RNA-seq identified 112 differentially expressed genes (DEGs) (74 up- and 38 down-regulated) (P≤0.05, |log2Fold Change|≥1) as induced by Vitamin C in mouse TM4 Sertoli cells. Gene enrichment analysis found multiple significantly enriched biological terms or pathways, including GO terms of regulation of response to biotic stimulus, homeostasis of number of cells, positive regulation of tumor necrosis factor production, GTPase activity, calmodulin binding etc., and KEGG signal pathways of calcium signal pathway, cGMP-PKG signaling pathway, steroid biosynthesis, arginine biosynthesis etc. 9 DEGs were selected for RT-qPCR validation and 8 of them showed the change trend consistent to RNA-seq. Taken together, Vitamin C (250µM, 36h) could modulate the functions of mouse TM4 Sertoli cells, through modifying the transcriptome to affect multiple signal pathways.

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2016-03-17 | E-GEOD-74471 | biostudies-arrayexpress

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Project description:The goat of this project is to explore M5 receptor regulation spermatogenesis. We tried to search the mechanism of M5 receptor regulation spermatogenesis. RNA-seq of C18-4 and TM4 cells samples from different groups: C3NC, M5 knockdown in C18-4, TM3NC, M5 knockdown in TM4 cells. The cell were transfected with virus with inhibition of M5 expression ( M5 gene knockdown).

2021-06-16 | GSE142618 | GEO

RNA-Seq of testicular peritubular myoid cells, other testicular cells, and other tissue smooth muscle-like cells

Project description:In addition to germ cell population, testes contain several types of somatic cells, including Sertoli cells, Leydig cells, and peritubular myoid (PTM) cells. PTM cells are a type of smooth muscle-like cells and have contraction ability. To identify a marker of PTM cells, we isolated primary cells and subsequently performed RNA-Seq of testicular peritubular myoid cells (PTM_1, PTM_2, PTM_3), testicular Sertoli cells (Sertoli_1, Sertoli_2, Sertoli_3), testicular Leydig cells (Leydig_1, Leydig_2, Leydig_3), testicular germ cells (Germ_1, Germ_2, Germ_3), vascular smooth muscle cells (VSMC_1, VSMC_2, VSMC_3), intestinal smooth muscle cells (ISMC_1, ISMC_2, ISMC_3), and tracheal smooth muscle cells (TSMC_1, TSMC_2, TSMC_3) using BGISEQ-500 platform (BGI, China).

2019-01-19 | GSE125303 | GEO

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