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LaminB1-DamID analysis of differentiating 3T3L1 mouse pre-adipocytes with knock-down of Tmem120a and Tmem120b

ABSTRACT: LaminB1-DamID analysis of differentiating 3T3L1 mouse pre-adipocytes with knock-down of Tmem120a and Tmem120b

PROVIDER: PRJNA632606 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11786157_1.fastq.gz	Fastqsanger.gz
	SRR11786157_2.fastq.gz	Fastqsanger.gz
	SRR11786158_1.fastq.gz	Fastqsanger.gz
	SRR11786158_2.fastq.gz	Fastqsanger.gz
	SRR11786159_1.fastq.gz	Fastqsanger.gz

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LaminB1-DamID analysis of differentiating 3T3L1 mouse pre-adipocytes with knock-down of Tmem120a and Tmem120b

Project description:Maps of genomic regions in proximity to the nuclear lamina were determined in undifferentiated 3T3L1 preadipocytes and 9 day differentiated (wild type, and under Tmem120a or Tmem120a/b knockdown) adipocytes using DamID with a Dam-Lamin B1-encoding lentivirus.

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Transcriptome analysis of differentiating 3T3L1 mouse pre-adipocytes with knock-down of Tmem120a and Tmem120b

Project description:The nuclear envelope transmembrane proteins (NETs) NET29a/Tmem120a and NET29b/Tmem120b are expressed or localise preferentially to the nuclear envelope in adipocytes. We knocked these proteins down using specific shRNAs and studied their effect in the diffentiation of the mouse 3T3L1 preadipocyte cell line.

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Analysis of differentiating 3T3L1 mouse pre-adipocytes with knock-down of Tmem120a and Tmem120b

Project description:Analysis of differentiating 3T3L1 mouse pre-adipocytes with knock-down of Tmem120a and Tmem120b

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Transcriptome analysis of differentiating 3T3L1 mouse pre-adipocytes with knock-down of Tmem120a and Tmem120b

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