Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing of whole SAS and FaDu spheroids transcriptomes treated with Hyperthermia (HT) and Radiation therapy (RT)

ABSTRACT: Next Generation Sequencing of whole SAS and FaDu spheroids transcriptomes treated with Hyperthermia (HT) and Radiation therapy (RT)

PROVIDER: PRJNA634094 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11817834_1.fastq.gz	Fastqsanger.gz
	SRR11817834_2.fastq.gz	Fastqsanger.gz
	SRR11817835_1.fastq.gz	Fastqsanger.gz
	SRR11817835_2.fastq.gz	Fastqsanger.gz
	SRR11817836_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Next Generation Sequencing of whole SAS and FaDu spheroids transcriptomes treated with Hyperthermia (HT) and Radiation therapy (RT)

Project description:By employing a global gene expression profiling, we performed the differential gene expression analysis and the analysis of the molecular pathways which are deregulated in Hyperthermia (HT) and Radiation therapy (RT) treated SAS and FaDu spheroids from the head-and-neck cancer cell lines. The RNA-Seq analysis was done on FaDu and SAS spheroids treated with clinically relevant HT dose of 42.5°C for 60 minutes alone or in combination of single dose irradiation of 7 and 10 Gy for FaDu and SAS, respectively. The main pathways and networks modulated by the treatments (0.5 h after treatment) were identiﬁed using Gene Ontology (GO) enrichment analysis.

2021-07-01 | GSE150922 | GEO

FaDu RT CRISPR Screen

Project description:CRISPR-Cas9 screen of FaDu Cas9 cells treated with fractionated RT.

2021-03-07 | GSE147084 | GEO

FaDu RT CRISPR Screen

Project description:FaDu RT CRISPR Screen

| PRJNA612971 | ENA

FaDu treated with citric buffer vs. rCTGF

Project description:FaDu treated with citric buffer vs. rCTGF FaDu treated with citric buffer for 24 hours FaDu treated with rCTGF 100 ng/mL for 24 hours

2013-01-01 | E-GEOD-30423 | biostudies-arrayexpress

Human tongue squamous cell carcinoma cell line SAS-Control vs. SAS-LYRIC knockdown stable clones global gene profiling

2016-02-28 | GSE44766 | GEO

Human tongue squamous cell carcinoma cell line SAS-Control vs. SAS-LYRIC knockdown stable clones global gene profiling

Project description:Transcriptional profiling of SAS cells transfected with pLKO.1-LYRIC shRNA-B expression vector (desinaged as B) and control SAS cells (transfected with pLKO.1 vector, designated as CTL). Goal was to determine the effects of LYRIC knockdown on global SAS cells gene expression. Two-condition experiment, SAS cells transfected with pLKO.1-LYRIC shRNA-B expression vector (desinaged as B) v.s. control SAS cells (transfected with pLKO.1 vector, designated as CTL). Biological replicates: 4 control replicates, 4 transfected replicates.

2016-02-28 | E-GEOD-44766 | biostudies-arrayexpress

SAS cells: control siRNA (siC) vs DDX3 siRNA (siD) transfected

Project description:Transcriptional profiling of SAS cells comparing siC-transfected SAS cells with siD-transfected SAS cells. The latter decreased proliferation and migration of SAS cells. Goal was to determine the DDX3-regulated transcripts.

2018-04-17 | GSE113183 | GEO

Molecular determinants of tumor response to hyperthermia

Project description:A deeper mechanistic understanding of response to hyperthermia in cancer cells in comparison between hyperthermia sensitive and resistant ovarian cancer cell lines

2016-12-31 | GSE77310 | GEO

Analysis of differentially expressed lncRNA, circRNA and mRNA between FaDu and cisplatin-resistant FaDu cell

Project description:Cisplatin is a common chemotherapeutic drug for hypopharyngeal cancer. But cisplatin-resistance of hypopharyngeal cancer is rarely explored. We cultured hypopharyngeal cancer cell (FaDu) and induced its cisplatin-resistant cell (FaDu/DDP4). The resistance index (RI) of FaDu/DDP4 was 2.828. Then we tested the differentially expressed genes (DEGs) between FaDu and FaDu/DDP4. DEGs contain 2388 lncRNAs, 1932 circRNAs, 745 mRNAs and 202 miRNAs. We used Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzed the DEGs. The differentially expressed 745 mRNAs were classified into 3 domains and 47 secondary GO terms. In KEGG pathway enrichment, “TNF signaling pathway”, “IL-17 signaling pathway” and “JAK-STAT signaling pathway” have greater enrich factors. And we drew the ceRNA networks of DEGs. 52 lncRNAs, 148 circRNAs, 155 mRNAs and 18 miRNAs were selected to draw the network. We noticed several potential targets (as miR-197-5p, miR-6808-5p, APOE, MMP1, S100A9 and CYP24A1). At last, we chose 8 miRNAs and 6 mRNAs for qRT-PCR to verify our microarray. In them, miR-197-5p, miR-6808-5p, APOE, MMP1, S100A9 and CYP24A1 might be potential genes inducing resistance.

2021-06-02 | GSE167396 | GEO

FaDu STING Isogenic RNA-Seq

Project description:RNA-Seq of FaDu Cas9 isogenic cells treated with fractionated RT.

2021-03-07 | GSE147085 | GEO