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Transcriptomic profilling of dental pericytes, an RNAseq approach [bulk RNA-Seq]

ABSTRACT: Transcriptomic profilling of dental pericytes, an RNAseq approach [bulk RNA-Seq]

PROVIDER: PRJNA634141 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11820223_1.fastq.gz	Fastqsanger.gz
	SRR11820223_2.fastq.gz	Fastqsanger.gz
	SRR11820224_1.fastq.gz	Fastqsanger.gz
	SRR11820224_2.fastq.gz	Fastqsanger.gz

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Transcriptomic profilling of dental pericytes, an RNAseq approach

Project description:Transcriptomic profilling of dental pericytes, an RNAseq approach

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Transcriptomic profilling of dental pericytes, an RNAseq approach [bulk RNA-Seq]

Project description:Profilling of pericytes derived from molar and incisors of XLacZ4 mice

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Transcriptomic profilling of dental pericytes, an RNAseq approach [scRNA-Seq]

Project description:Transcriptomic profilling of dental pericytes, an RNAseq approach [scRNA-Seq]

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Transcriptomic profilling of dental pericytes, an RNAseq approach [scRNA-Seq]

Project description:Profilling of pericytes derived from molar and incisors of XLacZ4 mice

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Transcriptomic profilling of dental pericytes, an RNAseq approach

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-07-13 | GSE150953 | GEO

Age-dependent RGS5 loss in pericytes induces cardiac dysfunction and fibrosis in the heart (Bulk RNA-SEQ)

Project description:Background. Ageing is one of the main risk factors of cardiovascular disease. Pericytes are capillary-associated mural cells involved in the maintenance and stability of the vascular network. In the heart, the consequences of ageing on cardiac pericytes are unknown. Methods. In this study, we have combined single nucleus RNA sequencing and histological analysis to determine the effects of ageing on cardiac pericytes. Furthermore, we have conducted in vivo and in vitro analysis of RGS5 loss of function and finally have perfomed pericytes-fibroblasts co-culture studies to understand the effect of RGS5 loss of function in pericytes on the neighbouring fibroblasts. Results. We showed that ageing reduces the pericyte area and coverage. Single nucleus RNA sequencing analysis further revealed that the expression of the Regulator of G protein signalling 5 (Rgs5) is reduced in old cardiac pericytes. In vivo and in vitro studies showed that the deletion of RGS5 induces morphological changes and a pro-fibrotic gene expression signature characterized by the expression of different extracellular matrix components and growth factors like TGFB2 and PDGFB in pericytes. Indeed, the culture of fibroblasts with the supernatant of RGS5 deficient pericytes induced their activation characterized by the increased expression of α smooth muscle actin in a TFGβ2 dependent mechanism. Conclusions. Our results identify RGS5 as a crucial regulator of pericyte function during cardiac ageing. The deletion of RGS5 causes cardiac dysfunction and induces myocardial fibrosis, one of the hallmarks of cardiac ageing.

2024-03-13 | E-MTAB-13833 | biostudies-arrayexpress

Global gene expression profile of human placental pericytes

Project description:The objective of this array was to determine the global gene expression profile of human placental pericytes for comparison with other publicly available arrays of pericytes and mesenchymal stromal cells isolated from various human tissues. Pericytes are critical cellular components of the microvasculature that play a major role in vascular development and pathologies, yet their study has been hindered by lack of a standardized method for their isolation and growth. Here we report a method for culturing human pericytes from a readily available tissue source, placenta, and provide a thorough characterization of resultant cell populations. We developed an optimized protocol for obtaining pericytes by outgrowth from microvessel fragments recovered after enzymatic digestion of human placental tissue. We characterized outgrowth populations by immunostaining, by gene expression analysis, and by functional evaluation of cells implanted in vivo. Our approach yields human pericytes that may be serially expanded in culture and that uniformly express the cellular markers NG2, CD90, CD146, α-SMA, and PDGFR-β, but lack markers of smooth muscle cells, endothelial cells, and leukocytes. When co-implanted with human endothelial cells into C.B-17 SCID/bg mice, human pericytes invest and stabilize developing human endothelial cell-lined microvessels. We conclude that our method for culturing pericytes from human placenta results in the expansion of functional pericytes that may be used to study a variety of questions related to vascular biology. Total RNA from three different pericyte isolations at subculture 1 was collected and examined for relative gene expression.

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Human Placenta profilling LTQ-OrbiTrap

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Expression profiling of lung pericytes treated with conditioned media from tumor cells

Project description:Lung pericytes treated with tumor-CM were processed and bulk RNA-seq analysis was performed.

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Differential gene expression in Jagged1 treated human dental pulp cells.

Project description:The present study aimed to determine mRNA expression profilling of indirect immobilized Jagged1 treated human dental pulp cells. Human dental pulp cells were seeded on indirect immobilized Jagged1 surface for 24 h. Cells on hFc immobilized surface was employed as the control. RNA sequencing was performed using NextSeq500, Illumina. Data were processed on FastQC and FastQ Toolkit and subsequently mapped with Homo sapiens hg38 using TopHat2. Mapped data were processed through Cufflink2 and Cuffdiff2. Results demonstrated 1,465 differentially expressed genes in Jagged1 treated cells compared with the control. Enriched pathway analysis revealed that Jagged1 treated cells upregulated genes mainly involved in extracellular matrix organization, disease, and signal transduction categories. However, genes related to cell cycle, DNA replication and DNA repair categories were downregulated. In conclusion, Jagged1 activates Notch signaling and regulates cell cycle pathway in hDPs.

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