Deciphering the molecular effects of non-ablative Er:YAG laser treatment in an in vitro model of the non-keratinized mucous membrane
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PROVIDER: PRJNA634480 | ENA |
REPOSITORIES: ENA
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Project description:This study aimed to investigate the molecular effects of non-ablative Er:YAG laser treatment using an in vitro model of the non-keratinized mucous membrane and to compare its molecular effects with other ablative and non-ablative laser systems. In dermatology, the use of non-ablative and ablative fractional lasers has become the gold standard treatment for a number of indications. Each of the individual laser types is advantageous for different types of indications due to its respective properties, but new technologies open up new fields of application for individual laser systems. Performing a comprehensive gene expression profiling we compared the gene regulatory effects of non-ablative Er:YAG laser with other non-ablative and ablative laser systems. In vitro 3D models have proven to be a reliable and reproducible tool to study the molecular biological effects of different laser settings.
2020-06-01 | GSE151041 | GEO
Project description:Efforts are increasingly aiming to develop in vitro models that can provide effective alternatives to in vivo experiments. The main aim of this study was the establishment of an in vitro model of the non-keratinized mucous membrane that can be used as a standardized tool to evaluate biological and therapeutic effects of pharmaceuticals for mucosal wound healing. Performing histological and immunofluorescence analyses with known differentiation markers we proved that our model mimics the two distinctive layers of the mucous membrane – the stratified squamous epithelium and the lamina propria. In our study we used our model to investigate molecular effects of a dexpanthenol-containing ointment that is widely used in the wound treatment of the oral mucosa. For that purpose our model exhibits a unique feature in that dexpanthenol and proliferation enhancing additives that may interfere with our studies are not required for the maintenance of the model culture. After setting standardized lesions with a CO2 laser, topical treatment with the dexpanthenol-containing ointment enhanced wound closure in our non-keratinized mucous membrane model compared to placebo and untreated controls. Furthermore, microarray analysis revealed that the treatment of our laser wounded model with the dexpanthenol-containing ointment evoked an upregulated expression of various genes related to accelerated wound healing. Overall, we verified that our mucous membrane model can be utilized in future to monitor ex vivo effects of various topical therapies on mucosa morphology, physiology, and gene expression. Our findings confirm the potential of the non-keratinized mucous membrane model as an in vitro tool for the replacement of pharmacological in vivo studies regarding mucosal wound healing.
2018-05-15 | GSE114378 | GEO
Project description:Efforts are increasingly aiming to develop in vitro models that can provide effective alternatives to in vivo experiments. The main aim of this study was the establishment of an in vitro model of the non-keratinized mucous membrane that can be used as a standardized tool to evaluate biological and therapeutic effects of pharmaceuticals for mucosal wound healing. Performing histological and immunofluorescence analyses with known differentiation markers we proved that our model mimics the two distinctive layers of the mucous membrane – the stratified squamous epithelium and the lamina propria. In our study we used our model to investigate molecular effects of a dexpanthenol-containing ointment that is widely used in the wound treatment of the oral mucosa. For that purpose our model exhibits a unique feature in that dexpanthenol and proliferation enhancing additives that may interfere with our studies are not required for the maintenance of the model culture. After setting standardized lesions with a CO2 laser, topical treatment with the dexpanthenol-containing ointment enhanced wound closure in our non-keratinized mucous membrane model compared to placebo and untreated controls. Furthermore, microarray analysis revealed that the treatment of our laser wounded model with the dexpanthenol-containing ointment evoked an upregulated expression of various genes related to accelerated wound healing. Overall, we verified that our mucous membrane model can be utilized in future to monitor ex vivo effects of various topical therapies on mucosa morphology, physiology, and gene expression. Our findings confirm the potential of the non-keratinized mucous membrane model as an in vitro tool for the replacement of pharmacological in vivo studies regarding mucosal wound healing.
2018-08-25 | GSE119035 | GEO
Project description:Non-ablative fractional laser can be used to treat photoaged skin by stimulating the breakdown and regeneration of dermal matrix constituents, such as collagen and elastic fibers. The objective of this study was to validate previously studied molecular mechanisms and explore yet uncharacterized biomarkers underlying the clinical efficacy of non-ablative fractional laser resurfacing.
2022-12-09 | GSE206495 | GEO
Project description:The purpose of this study was to evaluate comprehensive and sequential gene expressions in Er;YAG laser ablated bone, bur-drilled bone, and non-treated control bone to clarify the biological responses for bone healing.
2020-03-03 | GSE146182 | GEO
Project description:The purpose of this study was to evaluate comprehensive and sequential gene expressions in Er;YAG laser ablated bone, bur-drilled bone, and non-treated control bone to clarify the biological responses for bone healing.
2020-03-03 | GSE146181 | GEO
Project description:The purpose of this study was to evaluate comprehensive and sequential gene expressions in Er;YAG laser ablated bone, bur-drilled bone, and non-treated control bone to clarify the biological responses for bone healing.
2020-03-03 | GSE146180 | GEO
Project description:A number of reports showed that photo-bio-modulation by various lasers has favorable biological effects on cells. However, the effects of low-level Er:YAG laser irradiation on osteoblasts remain unclear. The purpose of this study was to evaluate the effects on proliferation and osteogenic differentiation of primary osteoblast-like cells by low-level Er:YAG laser irradiation. Osteoblast-like cells isolated from the calvariae of 3–5-day-old Wistar rats were irradiated by Er:YAG laser at energy fluences of 2.2, 3.3, or 4.3 J/cm2, respectively. After irradiation, cell surface temperature was measured and cell proliferation was evaluated by flow cytometry. Calcification was evaluated by measuring the Alizarin red S staining area after 7-day-culture with osteoinductive medium. Gene expressions in non-irradiated and laser-irradiated cells were evaluated by qPCR at 3, 6, and 12 hours after irradiation. Microarray analysis was performed to comprehensively evaluate gene expressions of non-irradiated cells and irradiated cells at 3.3 J/cm2 at 6 hours after irradiation. No pronounced increase of cell surface temperature was induced by low-level Er:YAG laser irradiation, and the Er:YAG laser irradiation did not affect osteoblast-like cell proliferation. Osteoblast-like cell calcification was significantly increased 7 days after Er:YAG laser irradiation at 3.3 J/cm2. Bglap expression was significantly increased in cells irradiated at 3.3 J/cm2 at 6 hours post-irradiation. Microarray analysis showed that irradiation at 3.3 J/cm2 caused up-regulation of inflammation-related genes and down-regulation of Wisp2. Gene set enrichment analysis also clarified enrichment of inflammation-related gene sets and Notch signaling pathway. In conclusion, Low-level Er:YAG laser irradiation enhanced calcification of primary osteoblast-like cells via enhanced Bglap expression and enriched Notch signaling pathway.
2022-02-28 | GSE146098 | GEO
Project description:The periocular region is challenging for cosmetic laser surgeons. Surgery and laser resurfacing have traditionally been used to correct periorbital lines and wrinkles. Although effective, the associated downtime with these methods has made many people reluctant to decide for such treatments. More recently, the non-ablative long-pulse 2940 nm Er:YAG laser is being used to improve the structure and function and hence the appearance of skin in the periorbital region. The objective of this study is to evaluate the safety and efficacy of long-pulse 2940 nm Er:YAG laser for non-ablative treatment of periorbital static wrinkles and skin laxity. This is a prospective analysis of 30 patients treated for periorbital rejuvenation using three sessions of non-ablative long-pulse Er:YAG laser over a 3-month period. All patients were assessed according to Fitzpatrick's classification of periorbital wrinkles to class I, II, or III and were treated with 2940 nm Er:YAG laser using a fluence of 3.75 J/cm2, a repetition rate of 1.7-2 Hz, and with the SMOOTH™ pulse mode (250 ms). The treatment sessions were performed on each patient, 4 weeks apart. Patient improvement was assessed before each laser session as well as at 12 months after the final treatment. Blind photographic evaluations were performed by three independent physicians using unlabeled before and after photos arranged in non-chronological order. Reviewers were asked to determine the before and after photos. Patients were asked to answer a questionnaire measuring satisfaction 4 weeks after each session, and to report any adverse reactions. There was statistically and clinically significant improvement in the Fitzpatrick classification of the periorbital wrinkles. Blinded evaluators correctly identified the before and after photos in all cases. All patients reported mild edema and erythema, which persisted for 1 to 2 days, and superficial peeling of the skin for 4 to 6 days after each laser treatment. No long-term adverse effects were reported. The non-ablative long-pulse 2940 nm Er:YAG laser seems to be a safe and effective treatment for periocular rejuvenation with minimal and tolerable adverse reaction. The improvement attained from the laser sessions was persisting after 1 year denoting the long-term efficacy of the procedure.
| S-EPMC8918125 | biostudies-literature
Project description:Novel human full-thickness three-dimensional non-keratinized mucous membrane model for pharmacological studies in wound healing
| PRJNA487804 | ENA