Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional comparison of in vitro and in vivo generated human dendritic cells

ABSTRACT: Transcriptional comparison of in vitro and in vivo generated human dendritic cells

PROVIDER: PRJNA634548 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11832600_1.fastq.gz	Fastqsanger.gz
	SRR11832600_2.fastq.gz	Fastqsanger.gz
	SRR11832601_1.fastq.gz	Fastqsanger.gz
	SRR11832601_2.fastq.gz	Fastqsanger.gz
	SRR11832602_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 88

Similar Datasets

Transcriptional comparison of in vitro and in vivo generated human dendritic cells

Project description:Transcriptional comparison of in vitro and in vivo generated human dendritic cells

| PRJNA608278 | ENA

Transcriptional comparison of in vitro generated human dendritic cells and peripheral blood circulating dendritic cells.

Project description:Transcriptional comparison of in vitro generated human dendritic cells and peripheral blood circulating dendritic cells.

| PRJNA603654 | ENA

Transcriptional comparison of in vitro generated human dendritic cells and peripheral blood circulating dendritic cells

Project description:Transcriptional comparison of in vitro generated human dendritic cells and peripheral blood circulating dendritic cells

| PRJNA634561 | ENA

Comparison of cortex generated either in vivo or in vitro from mouse ESC

Project description:Comparison of cortex generated either in vivo or in vitro from mouse ESC

| PRJNA304415 | ENA

Transcriptional comparison of in vitro and in vivo generated human dendritic cells

Project description:Classical dendritic cells (cDCs) are rare sentinel cells specialized in the regulation of adaptive immunity. Modelling cDC development is both crucial to study cDCs and harness their potential in immunotherapy. Here we present a novel in vitro differentiation approach using cord blood CD34+ hematopoietic stem and progenitor cells (HSPCs) co-cultured with bone marrow-derived murine mesenchymal cell line (MS5) engineered to express human FLT3L, SCF and CXCL12 (MS5_FS12). We also show that the engraftment of engineered mesenchymal stromal cells (MS5_FS12) together with CD34+ HSPCs creates an in vivo synthetic niche in the dermis of immunodeficient mice driving the differentiation of cDCs and CD123+AXL+CD327+ pre/AS-DCs. This approach provides a unique platform recapitulating the full spectrum of cDC subsets enabling their functional characterization in vivo.

2020-03-11 | GSE145803 | GEO

Transcriptional comparison of in vitro and in vivo generated human dendritic cells

Project description:Dendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC3) as an immediate precursor of inflammatory CD88-CD14+CD1c+CD163+FcεRI+ DC. DC3 develop via a specific pathway, independent from the cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors, and are activated by GM-CSF. As classical DC, but unlike monocytes, DC3 drove the activation of naïve T cells. In vitro, DC3 displayed a distinctive ability to prime CD8+ T cells expressing a tissue-homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor-b (TGF-β) signaling. In vivo, DC3 infiltrated luminal breast cancer primary tumors and DC3 infiltration correlated positively with CD8+CD103+CD69+ tissue-resident memory T cells. Altogether, these findings define DC3 as a lineage of inflammatory DC endowed with a strong potential to regulate tumor immunity.

2020-05-23 | GSE151073 | GEO

Transcriptional comparison of in vitro generated human dendritic cells and peripheral blood circulating dendritic cells.

2020-03-11 | GSE144435 | GEO

Transcriptional comparison of in vitro generated human dendritic cells and peripheral blood circulating dendritic cells

2020-05-23 | GSE151086 | GEO

Human XCR1+ Dendritic Cells Derived In Vitro from CD34+ Progenitors Closely Resemble Blood Dendritic Cells, Including Their Adjuvant Responsiveness, Contrary to Monocyte-Derived Dendritic Cells

Project description:Functional characterization of human dendritic cell subsets is limited due to the very low frequency of these cells in vivo. We developed an in vitro culture system for the simultaneous generation of XCR1+ DCs and MoDCs from cord blood CD34+ cells. Their global gene expression profiles at steady state and under activation, phenotypes, morphologies and responses to different TLR ligands where characterized and compared with those of their in vivo counterparts. The study demonstrated that the XCR1+ DCs generated in vitro from cord blood CD34+ cells are equivalent to blood XCR1+ DCs and also allowed a rigorous comparison of this DC subset with MoDC which are often considered as the reference model for DCs. Altogether, our results showed that in vitro generated XCR1+ DCs are a better model for the study of blood DC than the conventionally used MoDCs. The different dendritic cell subsets used for the study were either generated in vitro from cord blood CD34+ cells with recombinant human cytokines or isolated from peripheral blood. The subsets were purified by fluorescence-activated cell sorting and their responses to PolyI:C, LPS or R848 were studied including by gene expression profiling.

2014-07-23 | E-GEOD-57671 | biostudies-arrayexpress

Comparison of Leu3 binding in vitro and in vivo

Project description:1. Comparison of Leu3 binding in vitro and in vivo 2. comparison of nucleosome occupancy between with Leu3-binding and without Leu3-binding This SuperSeries is composed of the SubSeries listed below.

2006-09-08 | GSE5785 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data