Project description:An inhibitory effect of novobiocin on bop transcription was reported for the NRC1 strain of Hbt. salinarum ([17] Yang and DasSarma, 1990, [18] Yang et al, 1996), but its influence in strain R1 has not beeen known yet. We were interested to see the genome wide influences of novobiocin on transcription in Hbt. salinarum. Therefore, a DNA microarray analysis of the transcripts from R1 was performed, comparing cells grown in the presence or absence of novobiocin.
Project description:Previous work characterized TrmB as a global glucose responsive metabolic transcription factor in archaeal extremophiles. However, it remains unclear how TrmB dynamically regulates its ~100 metabolic enzyme-coding genes. Using a dynamic perturbation approach, we elucidate the topology of the metabolic GRN in Halobacterium salinarum. We assayed gene expression in a wild-type and trmB knockout strain before and immedeatly following glucose perturbation. Clustering dynamic gene expression patterns reveals that TrmB functions alone to regulate central metabolic enzyme-coding genes, but cooperates with various regulators to control peripheral metabolic pathways.
Project description:We set out to determine a) if histone in Halobacterium salinarum regulates transcription and b) whether the magnitude and extent of these changes matches those observed in organisms which use histone protein as their primary DNA packaging agent. To this end, gene expression data for a histone knock-out (Δura3ΔhpyA) strain versus parent (Δura3) were collected.