Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Facilitates Quantitative Analysis of Primary Keratinocytes and OSM-treated Primary Keratinocytes Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Primary Keratinocytes and OSM-treated Primary Keratinocytes Transcriptomes

PROVIDER: PRJNA635032 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11848644_1.fastq.gz	Fastqsanger.gz
	SRR11848644_2.fastq.gz	Fastqsanger.gz
	SRR11848645_1.fastq.gz	Fastqsanger.gz
	SRR11848645_2.fastq.gz	Fastqsanger.gz
	SRR11848646_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Next Generation Sequencing Facilitates Quantitative Analysis of Primary Keratinocytes and OSM-treated Primary Keratinocytes Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) between Primary Keratinocytes and OSM-treated Primary Keratinocytes Methods: mRNA profiles of Primary Keratinocytes and OSM-treated Primary Keratinocytes were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.

2021-03-18 | GSE151174 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of HaCaT and OSM-treated HaCaT Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) between HaCaT cell and OSM-treated HaCaT cells Methods: mRNA profiles of HaCaT cells and OSM-treated HaCaT cells were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.

2021-03-18 | GSE150994 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of HaCaT and OSM-treated HaCaT Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of HaCaT and OSM-treated HaCaT Transcriptomes

| PRJNA634341 | ENA

VZV infection of primary keratinocytes

Project description:VZV infection of primary keratinocytes

| PRJEB4204 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Stat3f/f K5cre Skin Keratinocytes Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Stat3f/f K5cre Skin Keratinocytes Transcriptomes

| PRJNA768791 | ENA

Oncostatin M (OSM) and Leukemia Inhibitory factor (LIF) treatment of osteoblasts via LIF and OSM receptors

Project description:Oncostatin M (OSM) and Leukemia Inhibitory Factor (LIF) signal within cells via the gp130 (Il6st) coreceptor bound either to the LIF receptor (LIFR) or the oncostatin M receptor (OSMR), but whether murine OSM can act through both receptors is controversial. Both LIF and OSM stimulate bone formation, inhibit adipocyte differentiation, and promote osteoclast differentiation, but our earlier work suggested this may depend on the receptor subtype used. This project aimed to identify those gene targets regulated by murine OSM via OSMR and LIFR by using wild type and OSMR null primary osteoblasts. Cells were differentiated to their most mature state (i.e. osteocytes) because the only prior target gene known to be regulated by murine OSM via the LIFR was an osteocyte-specific gene, sclerostin.

2016-08-17 | GSE83418 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Osmr-/- Epidermal keratinocytes Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare epidermal keratinocytes transcriptome profiling (RNA-seq) between wild type and Osmr-/- mice Methods: Epidermal keratinocytes mRNA profiles of wild-type (WT) and Oncostatin M receptor (Osmr−/−) mice were generated by deep sequencing, in triplicate. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.

2021-03-18 | GSE150884 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Osmr-/- Epidermal keratinocytes Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Osmr-/- Epidermal keratinocytes Transcriptomes

| PRJNA633911 | ENA

microRNA profiling of primary human keratinocytes and primary human dermal fibroblasts

Project description:In search for factors, overexpression of which in human dermal fibroblasts causes direct conversion to cells similar to keratinocytes, micro RNA expression profiles of human primary keratinocytes and human primary dermal fibroblasts are investigated. Skin samples obtained from 3 different sites of 1 subject were used for establishment of 3 primary keratinocytes and 3 primary dermal fibroblasts. Thus obtained 3 primary keratinocytes and primary dermal fibroblasts underwent micro RNA profiling.

2016-09-30 | E-MTAB-5055 | biostudies-arrayexpress

Expression data from human primary keratinocytes

Project description:The genetic expression profile of a Wnt signal agonist, BIO, was evaluated in human primary keratinocytes. Accelerating scaling up of primary keratinocytes benefits skin autografts for severely burned patients. Wnt signal, a conserved pathway controlling cell cycle and morphogenesis of embryo, has been postulated to promote the cell proliferation and tumorigenesis in adult. Here, the effects of Wnt signal on the growth of interfollicular keratinocytes were investigated. We demonstrated that recombinant Wnt3a significantly promoted the primary keratinocyte growth at a low cell density. A well-characterized GSK-3beta inhibitor, BIO, activated the Wnt signals and also enhanced the colony formation of keratinocytes dose-dependently. Gene expression profile of the BIO-treated keratinocytes revealed the linkage of the BIO with the cell mitosis and indicated that epithelial cell adhesion molecule (EpCAM), a Wnt target gene, was upregulated. Comparing to the EpCAM- keratinocytes, the EpCAM+ cells showed higher proliferation rate and efficacy of the colony formation. Especially, inhibiting the EpCAM expression by shRNA attenuated the proliferation effect of BIO and the growth advantage of the EpCAM+ keratinocytes. These evidences emphasize the positive role of canonical Wnt and EpCAM on the regulation of cell growth and self-renewal for human keratinocytes.

2012-10-01 | E-GEOD-40233 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data