Project description:Gram-stain-negative, strictly aerobic, non-spore-forming, non-motile, and rod-shaped bacterial strains, designated NC18T and NC20, were isolated from the sediment near-vertical borehole effluent originating 714 m below the subsurface located in the Soudan Iron Mine in Minnesota, USA. The 16S rRNA gene sequence showed that strains NC18T and NC20 grouped with members of the genus Martelella, including M. mediterranea DSM 17316T and M. limonii YC7034T. The genome sizes and G + C content of both NC18T and NC20 were 6.1 Mb and 61.8 mol%, respectively. Average nucleotide identity (ANI), the average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values were below the species delineation threshold. Pan-genomic analysis showed that NC18T, NC20, M. mediterranea DSM 17316T, M. endophytica YC6887T, and M. lutilitoris GH2-6T had 8470 pan-genome orthologous groups (POGs) in total. Five Martelella strains shared 2258 POG core, which were mainly associated with amino acid transport and metabolism, general function prediction only, carbohydrate transport and metabolism, translation, ribosomal structure and biogenesis, and transcription. The two novel strains had major fatty acids (>5%) including summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C19:0 cyclo ω8c, C16:0, C18:1 ω7c 11-methyl, C18:0, and summed feature 2 (C12:0 aldehyde and/or iso-C16:1 I and/or C14:0 3-OH). The sole respiratory quinone was uniquinone-10 (Q-10). On the basis of polyphasic taxonomic analyses, strains NC18T and NC20 represent novel species of the genus Martelella, for which the name Martelella soudanensis sp. nov. is proposed. The type strain is NC18T (=KTCT 82174T = NBRC 114661T).
Project description:The pyrite mine of Lousal, Grândola, Portugal, was discovered in 1882 and extensively exploited from 1900 until its closure in 1988 when the mining ores were not viable economically. Aiming at becoming a cultural-touristic center, the mining area was rehabilitated raising a Mining Museum and a Science Centre along with the reopening of a mining gallery for public access. In recent times, aerobiological studies on subterranean environments such as caves, mines and archeological necropolis revealed to be an important source of novel bacteria, from which their biotechnological potential are under study. Here we present a Pseudomonas sp. isolated from the mine air with potential secondary metabolite biosynthesis, resistance to antibiotics and virulence factors.