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Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [bis-seq]

ABSTRACT: Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [bis-seq]

PROVIDER: PRJNA635876 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11874066.fastq.gz	Fastqsanger.gz
	SRR11874067.fastq.gz	Fastqsanger.gz
	SRR11874068.fastq.gz	Fastqsanger.gz
	SRR11874069.fastq.gz	Fastqsanger.gz
	SRR11874070.fastq.gz	Fastqsanger.gz

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Similar Datasets

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro.

Project description:Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro.

| PRJNA635873 | ENA

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [bis-seq]

Project description:Primordial germ cells (PGCs) are the founding population of the germ cell lineage that undergo a multistep process to generate spermatozoa or oocytes. Establishing an appropriate culture system for PGCs is a key challenge in reproductive biology. By a chemical screening using mouse PGC-like cells (mPGCLCs), which were induced from mouse embryonic stem cells, we reported previously that forskolin and rolipram synergistically enhanced the proliferation/survival of mPGCLCs with an average expansion rate of ∼20-fold. In the present study, we evaluated other chemicals or cytokines to see whether they would improve the current mPGCLC culture system. Among the chemicals and cytokines examined, in the presence of forskolin and rolipram, cyclosporin A (CsA) and fibroblast growth factors (FGFs: FGF2 and FGF10) effectively enhanced the expansion of mPGCLCs in vitro (∼50-fold on average). During the expansion by CsA or FGFs, mPGCLCs comprehensively erased their DNA methylation to acquire a profile equivalent to that of gonadal germ cells in vivo, while maintaining their highly motile phenotype as well as their transcriptional properties as sexually uncommitted PGCs. Importantly, these mPGCLCs robustly contributed to spermatogenesis and produced fertile offspring. Furthermore, mouse PGCs (mPGCs) cultured with CsA ex vivo showed transcriptomes and DNA methylomes similar to those of cultured mPGCLCs. The improved culture system for mPGCLCs/mPGCs would be instructive for addressing key questions in PGC biology, including the mechanisms for germ cell migration, epigenetic reprogramming, and sex determination of the germline.

2021-02-16 | GSE151445 | GEO

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [RNA-seq]

Project description:Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [RNA-seq]

| PRJNA635877 | ENA

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [RNA-seq]

2021-02-16 | GSE151444 | GEO

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro.

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-02-16 | GSE151446 | GEO

Murine Back Skin: Control vs. Cyclosporin A-treated

Project description:We analyzed the gene expression at the onset of hair growth induced by cyclosporin A (CsA), a well-known hair growth inducer, with DNA microarray, and unveiled the step-by-step progression of hair growth. KEYWORDS: Cyclosporin A, Cyclosporine A, Ciclosporin A

2009-12-24 | GSE16739 | GEO

Renal Cyclosporin-A (CsA) toxicity

Project description:Renal Cyclosporin-A (CsA) toxicity

| PRJNA437288 | ENA

Secretomics of Jurkat cells treated with sanglifehrin A or cyclosporin A

Project description:Proteins secreted from Jurkat cells upon treatment with sanglifehrin A or cyclosporin A were profiled.

2024-10-17 | PXD031010 | Pride

Brachypodium distachyon cultivar:Bis-1

Project description:Brachypodium distachyon cultivar:Bis-1 Genome sequencing

| PRJNA337153 | ENA

FGF inhibition directs BMP4-mediated differentiation of Human Embryonic Stem Cells to syncytiotrophoblast

Project description:Bone morphogenetic protein (BMP) signaling is known to support differentiation of human embryonic stem cells (hESCs) into mesoderm and extraembryonic lineages, whereas other signaling pathways can largely influence this lineage specification. Here, we set out to reinvestigate the influence of ACTIVIN/NODAL and fibroblast growth factor (FGF) pathways on the lineage choices made by hESCs during BMP4-driven differentiation. We show that BMP activation, coupled with inhibition of both ACTIVIN/NODAL and FGF signaling, induces differentiation of hESCs, specifically to M-NM-2hCG hormone-secreting multinucleated syncytiotrophoblast and does not support induction of embryonic and extraembryonic lineages, extravillous trophoblast, and primitive endoderm. It has been previously reported that FGF2 can switch BMP4-induced hESC differentiation outcome to mesendoderm. Here, we show that FGF inhibition alone, or in combination with either ACTIVIN/NODAL inhibition or BMP activation, supports hESC differentiation to hCG-secreting syncytiotrophoblast. We show that the inhibition of the FGF pathway acts as a key in directing BMP4-mediated hESC differentiation to syncytiotrophoblast. Human embryonic Stem Cells (hESCs) were treated under defined conditions (N2B27) with BMP4 (B), SB431542 (SB) (ACTIVIN/NODAL inhibitor), SU5402 (SU) (FGFR1 inhibitor), FGF2 (F) either alone or in various combinations as mentioned, followed by isolation of total RNA.

2012-10-17 | E-GEOD-30125 | biostudies-arrayexpress

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