Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of QPRT silencing in breast cancer cells

ABSTRACT: Transcriptome analysis of QPRT silencing in breast cancer cells

PROVIDER: PRJNA636197 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11885119.fastq.gz	Fastqsanger.gz
	SRR11885119_1.fastq.gz	Fastqsanger.gz
	SRR11885119_2.fastq.gz	Fastqsanger.gz
	SRR11885120.fastq.gz	Fastqsanger.gz
	SRR11885120_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Transcriptome analysis of QPRT silencing in breast cancer cells

Project description:We identified quinolinate phosphoribosyltransferase (QPRT) as a prognostic factor in breast cancer. Transcription profiling analysis was performed in breast cancer cells transfected with control or QPRT shRNA.

2020-06-01 | GSE151521 | GEO

FAM83H-AS1 silencing in MCF7 breast cancer cells

Project description:FAM83H-AS1 silencing in MCF7 breast cancer cells

| PRJNA558978 | ENA

Proteomic analysis of the secretome from 4T1 breast cancer cells upon Stat3 silencing.

Project description:Stat3 has been acknowledged as an oncogene that has dual protumorigenic activity in a cell-intrinsic way, by promoting cancer cell proliferation and survival and in a paracrine mode, acting as suppressor of immune cell attack. We demonstrated that Stat3 silencing with a small interfering RNA (siRNA) induced a senescence program in Stat3-addicted cancer cells and leads to the production of a senescence associated secretory phenotype (SASP) that has several antitumor properties. The composition of the SASP induced by Stat3 silencing is poorly characterized. The goal of the project was to analyze the protein content in the secretome released by the mouse breast cancer cell line 4T1 after Stat3 silencing with a small interfering RNA.

2020-01-21 | PXD013948 | Pride

Analysis of breast cancer cells

Project description:Analysis of breast cancer cells

| PRJNA1010787 | ENA

Transcriptome analysis of CHI3L1 silencing in thyroid cancer cells

Project description:Transcriptome analysis of CHI3L1 silencing in thyroid cancer cells

| PRJNA605952 | ENA

FAM83H-AS1 silencing in MCF7 breast cancer cells

Project description:We silenced FAM83H-AS1 shRNAs in cell line MCF7 carried a ~75% silencing compared to thenegative control (NC). We evaluated the role of FAM83H-AS1 on oncogenic phenotypes in the MCF7 breast cancer cell line model. The knockdown of FAM83H-AS1 was achieved with ~75% of silencing efficiency. A complete transcriptomic analysis after silencing of FAM83H-AS1 revealed an impact on the global expression.

2020-12-12 | GSE135466 | GEO

Silencing ferrochelatase in breast cancer cells MDA-MB-231

Project description:Silencing ferrochelatase in breast cancer cells MDA-MB-231

| PRJNA560254 | ENA

RHBDD2 silencing and gene expression profiling of breast cancer cells

Project description:In previous studies, we identified a distantly related rhomboid homologue gene known as RHBDD2 (Rhomboid domain containing 2) to be markedly overexpressed in the advanced stages of the breast and colorectal cancer diseases. In order to identify RHBDD2 modulated pathways, we analyzed two breast cancer cell lines (MCF7 and T47D) from control and RHBDD2-siRNA transient gene silencing followed by gene expression profiling analysis using the whole genome Toray 3D-GeneTM Human Oligo Chip. Statistical analysis of the Toray's 3D gene expression profiling data identified 566 commonly differentially expressed genes in association to the RHBDD2 knockdown in both breast cancer cell lines. Among the statistically significant over-represented biological process, we found the apoptosis, cell cycle and response to DNA damage process related genes. In addition, categories of genes found in the ubiquitin-proteasome and oxidative phosphorylation were also highly enriched related genes in the commonly deregulated gene list. We further used a lentivirus-based system (shRNA-pLKO.1) for stable silencing of RHBDD2 mRNA in the T47D breast cancer cell line. Using a staurosporine-induced apoptosis model, we demonstrate that RHBDD2 abrogation resulted in an apoptosis-resistant phenotype of T47D breast cancer cell line. These data are in line with a recent study, suggesting that RHBDD2 expression could be up-modulated in response to 5FU-induced apoptosis in colorectal cancer cells. Taken together, these data suggest that RHBDD2 could be involved in the modulation of the programmed cell death in cancer cells. In order to analyze differential gene expression profiling of RHBDD2 silencing and control cells, total RNA was isolated from replicate experiments from two breast cancer cell lines (MCF7 and T47D) derived from the negative control-siRNA and the RHBDD2-siRNA treatments in duplicate experiments.

2013-04-10 | E-GEOD-43015 | biostudies-arrayexpress

Genome-wide RNA analysis of the effects of MASTL silencing in breast cancer cells

Project description:Genome-wide RNA analysis of the effects of MASTL silencing in breast cancer cells

| PRJNA545096 | ENA

Transcriptional profile of recurrent breast cancer cells with RIPK3 silencing

Project description:Transcriptional profile of recurrent breast cancer cells with RIPK3 silencing

| PRJNA512881 | ENA