Understanding seasonal weight loss tolerance in dairy goats: a transcriptomics approach
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA636512 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR11905278_1.fastq.gz | Fastqsanger.gz | |||
| SRR11905278_2.fastq.gz | Fastqsanger.gz | |||
| SRR11905279_1.fastq.gz | Fastqsanger.gz | |||
| SRR11905279_2.fastq.gz | Fastqsanger.gz | |||
| SRR11905280_1.fastq.gz | Fastqsanger.gz |
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Project description:Seasonal weight loss is one of the most important limitations to ruminant production in drought-prone regions. In these regions and under the dry season, animals may lose up to 30% of their body weight, conditioning production performance. It is of the utmost importance to understand the physiological basis of tolerance to SWL, particularly by contrasting breeds that are susceptible and tolerant to SWL. In this study, transcriptomics was used to describe the gene expression effects of feed restriction in dairy goat of two breeds with different SWL tolerance: Majorera (tolerant) and Palmera (susceptible). Two control and two restricted groups per breed were established: Majorera Control (adequately fed; MC; n=4), Palmera Control (adequately fed; PC; n=6), Majorera Restricted (feed restricted; ME; n=5) and Palmera Restricted (feed restricted; PE; n=4). On day 22 of the trial, mammary gland biopsies were collected for transcriptomics analysis. Results were obtained using Next Generation RNA sequencing and validation using qPCR.
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Project description:BackgroundSeasonal weight loss (SWL) is a very important limitation to the production of ruminants in the Mediterranean and Tropical regions. In these areas, long dry seasons lead to poor pastures with low nutritional value. During the dry season, ruminants, particularly those raised in extensive production systems, lose around 30% of their body weight. Seasonal weight loss has important consequences on animal productive performance and health. In this study, RNA sequencing was used to characterize feed restriction effects in dairy goat of 2 breeds with different SWL tolerance: Majorera (tolerant) and Palmera (susceptible). Nine Majorera and ten Palmera goats were randomly distributed in a control and a restricted group: Majorera Control (adequately fed; MC; n = 4), Palmera Control (adequately fed; PC; n = 6), Majorera Restricted (feed restricted; ME; n = 5) and Palmera Restricted (feed restricted; PE; n = 4). On day 22 of the trial, mammary gland biopsies were collected for transcriptomics analysis.ResultsFrom these samples, 24,260 unique transcripts were identified. From those, 82 transcripts were differentially expressed between MC and ME, 99 between PC and PE, twelve between both control groups and twenty-nine between both restricted groups.ConclusionsFeed restriction affected several biochemical pathways in both breeds such as: carbohydrate and lipid transport; intracellular trafficking, RNA processing and signal transduction. This research also highlights the importance or involvement of the genes in tolerance (ENPP1, S-LZ, MT2A and GPNB) and susceptibility (GPD1, CTPS1, ELOVL6 and NR4A1) to SWL with respectively higher expression in the Majorera restriced group and the Palmera restricted group in comparison to the control groups. In addition, results from the study may be extrapolated to other dairy ruminant species.
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