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ABSTRACT: RNA sequencing of human gastric cancer cell line (AGS) 'DDX53'

PROVIDER: PRJNA636928 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11912575_1.fastq.gz	Fastqsanger.gz
	SRR11912575_2.fastq.gz	Fastqsanger.gz
	SRR11912576_1.fastq.gz	Fastqsanger.gz
	SRR11912576_2.fastq.gz	Fastqsanger.gz
	SRR11912577_1.fastq.gz	Fastqsanger.gz

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Project description:RNA-seq of human gastric cancer cell line (AGS)

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lnc-PLCB1 is stabilized by METTL14 induced m6A modification and inhibits Helicobacter pylori mediated gastric cancer by degrading DDX21

Project description:To determine the role of m6A modification in H.pylori-mediated gastric cancer, we performed RNA methylation immunoprecipitation sequencing (MeRIP-seq) after infecting gastric cancer cell line AGS with H. pylori standard strain Hp26695 at MOI=100 for 12h. The sequencing results include three parts: mRNA, lncRNA, and circRNA.

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Project description:In this study, we treated the gastric cancer cell line AGS with PBS and Helicobacter pylori to perform RNA-seq analysis. A total of 18,308 different circRNA candidates were obtained in the experiment.Compared with the control, 101 significantly differentially expressed circRNAs were identified in the AGS cells infected with H. pylori, including 84 upregulated circRNAs and 17 downregulated circRNAs.Then, circMAN1A2 with the most significant expression difference was selected according to the sequencing results to study the epigenetic mechanism of H. pylori-induced gastric carcinogenesis.

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Project description:Background Mitogen-activated protein kinase 1 (MAPK1) has independent functions of phosphorylating histones as a kinase and directly binding the promoter regions of genes to regulate gene expression as a transcription factor. Previous studies identified elevated expression of MAPK1 in human gastric cancer, which is associated with its role as a kinase, facilitating gastric cancer cell migration and invasion. However, being a transcription factor, how MAPK1 binds its target genes and whether it modulated related gene expressions in gastric cancer remains unclear. Results Here, we integrated biochemical assays (protein interactions and chromatin immunoprecipitation (ChIP)), cellular analysis assays (cell proliferation and migration), RNA sequencing, ChIP sequencing, and clinical analysis to investigate the potential genomic recognition patterns of MAPK1 in a human gastric adenocarcinoma cell-line (AGS) and to uncover its regulatory effect on gastric cancer progression. We confirmed that MAPK1 promotes AGS cells invasion and migration by regulating the target genes in controversial directions, up-regulating seven target genes (KRT13, KRT6A, KRT81, MYH15, STARD4, SYTL4, and TMEM267) and down-regulating one gene (FGG). Among them, five genes (FGG, MYH15, STARD4, SYTL4, and TMEM267) were first associated with cancer procession, while the other three (KRT81, KRT6A, and KRT13) have been previously confirmed to be related to cancer metastasis and migration. Conclusion Our data showed that MAPK1 binds to the promoter regions of these target genes to control their transcription, hence encouraging AGS cell motility and invasion. Our research broadened the understanding of the regulatory roles of MAPK1, enriched the knowledge of transcription factors, and provided novel candidates for cancer therapeutics.

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