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Transcriptional response of turkey blood leukocytes challenged with Salmonella enterica serovar Typhimurium UK1

ABSTRACT: Transcriptional response of turkey blood leukocytes challenged with Salmonella enterica serovar Typhimurium UK1

PROVIDER: PRJNA637601 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11935264_1.fastq.gz	Fastqsanger.gz
	SRR11935264_2.fastq.gz	Fastqsanger.gz
	SRR11935265_1.fastq.gz	Fastqsanger.gz
	SRR11935265_2.fastq.gz	Fastqsanger.gz
	SRR11935266_1.fastq.gz	Fastqsanger.gz

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Transcriptional response of turkey blood leukocytes challenged with Salmonella enterica serovar Typhimurium UK1

Project description:Salmonella is one of most common causes of bacterial foodborne disease and consumption of contaminated poultry products, including turkey, is one route of exposure. Minimizing colonization of commercial turkeys with Salmonella could reduce the incidence of Salmonella-associated human foodborne illness. Understanding host responses to these bacteria could lead to potential strategies to minimize colonization and thus food safety risk. In this study, we evaluated bacterial load and blood leukocyte transcriptomic responses of 3-week-old turkeys challenged with the Salmonella enterica serovar Typhimurium (S. Typhimurium) UK1 strain. Turkeys (n = 8/dose) were inoculated with 108 or 1010 colony forming units (CFU) of S. Typhimurium UK1 and fecal shedding and tissue colonization were measured across multiple days post inoculation (dpi). Fecal shedding was 1-2 log10 higher in the 1010 CFU group than the 108 CFU group, but both doses effectively colonized the crop, spleen, ileum, cecum, colon, bursa of Fabricius and cloaca without causing any overt clinical signs in either group of birds. Blood leukocytes were isolated from a subset of the birds (n =3-4/dpi) both pre-infection (0 dpi) and 2 dpi with 1010 CFU and their transcriptomic responses assayed by RNA-sequencing (RNA-seq). After 2 dpi, 647 genes had significant differential expression (DE), including large increases in expression of immune genes such as CCAH221, IL4I1, LYZ, IL13RA2, IL22RA2, and ACOD1. IL1B was predicted as a major regulator of DE in these leukocytes and this DE was predicted to activate cell migration, phagocytosis and proliferation, and to impact the STAT3 and toll-like receptor pathways. These data revealed genes and pathways by which turkey blood leukocytes responded to the pathogen and can provide potential targets for developing intervention strategies or diagnostic assays to mitigate S. Typhimurium in turkeys.

2021-12-31 | GSE151896 | GEO

Expression analysis of Salmonella enterica serovar Typhimurium UK1 delta-iacP mutant

Project description:Investigation of whole genome gene expression level changes in a Salmonella enterica serovar Typhimurium UK1 delta-iacP mutant, compared to the wild-type strain. IacP is resoponsible for the secretion of virulence effector proteins via the type III secretion system, thereby contributing the virulence of S. Typhimurium. The mutants analyzed in this study are further described in Kim et al. 2011. Role of Salmonella Pathogenicity Island 1 Protein IacP in Salmonella enterica Serovar Typhimurium Pathogenesis. Infection and Immunity 79(4):1440-1450 (PMID 21263021). A chip study using total RNA recovered from two separate wild-type cultures of Salmonella enterica serovar Typhimurium UK1 and two separate cultures of a mutant strain, Salmonella enterica serovar Typhimurium UK1 delta-iacP. Each chip measures the expression level of 4,302 genes from Salmonella enterica serovar Typhimurium.

2012-04-29 | E-GEOD-34663 | biostudies-arrayexpress

Expression analysis of Salmonella enterica serovar Typhimurium UK1 delta-iacP mutant

2012-04-30 | GSE34663 | GEO

Transciptome analysis of Salmonella enterica serovar Typhimurium UK1 delta-rsiM at oxidative stress

Project description:Salmonella has various mechanisms of small RNA-mediated gene regulation. In Salmonella enterica serovar Typhimurium, a novel intergenic transcript RsiM is involved in oxidative stress response which functions as one of the powerful antimicrobials in macrophage innate immunity. We note that the M-bM-^HM-^FrsiM mutant is sensitive to hydrogen peroxide (5.0mM). This finding provides insights into the function of RsiM as a regulator of oxidative stress response. UK1 wild-type and M-bM-^HM-^FrsiM strains were grown in LB medium at 37M-bM-^DM-^C to an OD600 of 0.6, and then subjected to oxidative stress (5.0 mM hydroperoxide) for 30 min. Total RNA was extracted from three biological replicates of wild-type and M-bM-^HM-^FrsiM mutant.

2012-06-30 | E-GEOD-37626 | biostudies-arrayexpress

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Oxidative Stress and Reduced Responsiveness of Challenged Circulating Leukocytes Following Pulmonary Instillation of Metal-Rich Particulate Matter in Rats

2014-06-15 | GSE58197 | GEO

Transciptome analysis of Salmonella enterica serovar Typhimurium UK1 delta-raoN at oxidative stress

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Oxidative Stress and Reduced Responsiveness of Challenged Circulating Leukocytes Following Pulmonary Instillation of Metal-Rich Particulate Matter in Rats

Project description:Exposure to welding fume consists of a mixed exposure of metal-rich particulate matter with gases (ozone, carbon monoxide) and/or vapors (VOCs). Data suggests that welders are immune compromised. Given the inability of pulmonary leukocytes to properly respond to a secondary infection, the question arose concerning how far upstream the dysfunction persisted. Our aim was to evaluate circulating leukocyte functionality after welding fume exposure. The results showed that welding fume exposure has rapid effects on the circulating leukocyte population as identified by relative mRNA expression changes. Instillation of MMA-SS reduced inflammatory protein production of circulating leukocytes when challenged with the secondary stimulus LPS. The effects were not related to transcription, but were observed in conjunction with oxidative stress. Rats were intratracheally instilled (ITI) with phosphate buffered saline or 2 mg of welding fume collected from a manual metal arc-stainles steel weld. Rats were sacrificed 4 and 24 h post-exposure and whole blood was collected. Whole blood was used for cellular differential counts, RNA isolation with subsequent microarray and Ingenuity Pathway Analysis, and secondary stimulation with lipopolysaccharide utilizing TruCulture technology from Myriad/Rules Based Medicine. Also, mononuclear cells were isolated 24 h post-exposure to measure oxidative stress by flow cytometry and confocal microscopy. Whole blood collected 4 and 24 h post-exposure was challenged (a paired non-stimulant (null) and stimulant (LPS) tube) for 24 h using Truculture technology from Myriad/ RBM. After the challenge, cells were recovered using RNA later for subsequent RNA isolation and microarray.

2014-06-15 | E-GEOD-58197 | biostudies-arrayexpress

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