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ABSTRACT: Temporal and cultivar-specific effects on fungal diversity

PROVIDER: PRJNA638263 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11965405_1.fastq.gz	Fastqsanger.gz
	SRR11965405_2.fastq.gz	Fastqsanger.gz
	SRR11965406_1.fastq.gz	Fastqsanger.gz
	SRR11965406_2.fastq.gz	Fastqsanger.gz
	SRR11965407_1.fastq.gz	Fastqsanger.gz

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Project description:Fungal effectors are key determinants of disease development, promoting pathogen growth and spreading in plant tissues. In the wheat/Fusarium graminearum (Fg) interaction determining the Fusarium Head Blight disease (FHB), the molecular bases of Fg aggressiveness remain widely unknown. The aim of this work is to provide a better understanding of Fusarium graminearum aggressiveness determinism during its interaction with bread wheat. Phenotyping in controlled conditions of three fungal strains was carried out on three wheat cultivars contrasted by their susceptibility to FHB. Qualitative and quantitative characterizations of the three strain's whole proteomes expressed in planta have been performed with special interest on effectors and proteins coded by Fg aggressiveness-related genes. Considering all fusaried wheat samples, this experimentation identified 615 unique Fg proteins, including 583 identified in all samples. Furthermore, 71 putative Fg effectors were found in the identified proteins. Among all the fungal proteins quantified, nearly 76% showed significant abundance differences between the Fg strains. Two-way ANOVA were computed to evaluate the contribution of the "Strain" and "Cultivar" factors on the Fg protein abundances. Abundances that were deemed significant to each factor enabled a categorization of the proteins according to the factor(s) that drive(s) the abundance differences. This included: (i) Fg proteins whose abundance differences were specifically explained by the genetic background of the strain (Strain factor, Strain_effect proteins), (ii) Fg proteins whose abundance differences were only explained by the genetic background of the host plant (Cultivar factor, Cultivar_effect proteins), (iii) Fg proteins whose abundance differences were explained by both factors (Strain+Cultivar proteins), and (iv) Fg proteins whose abundance differences observed between strains were dependent on the host cultivar (Strain×Cultivar proteins). Considering each factor individually, a total of 139 Strain_effect proteins, 82 Cultivar_effect proteins, 117 Strain+Cultivar proteins and 2 Strain×Cultivar proteins were identified.

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