Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Epidemiology and Genomics Features of Klebsiella spp especially ST11-K64 CR-hvKP Outbreaks

PROVIDER: PRJNA638288 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR20999547_1.fastq.gz	Fastqsanger.gz
	SRR20999548.fastq.gz	Fastqsanger.gz
	SRR21808492_1.fastq.gz	Fastqsanger.gz
	SRR21808492_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 5

Similar Datasets

Project description:ST11-KL47 CR-hvKP

| PRJNA874350 | ENA

Project description:Clinical CR-hvKP ST11 strains collection

| PRJNA824258 | ENA

Project description:Clinical CR-hvKP ST11 strains collection

| PRJNA820335 | ENA

Project description:Non-mucoid CR-hvKp strain

| PRJNA819844 | ENA

RNA interactome profiling in hypervirulent Klebsiella pneumoniae reveals small RNA ArcZ as an inhibitor of mucoviscosity and virulence

Project description:Hypervirulent Klebsiella pneumoniae (HvKP) is an emerging human pathogen causing invasive infection in immune-competent hosts. The hypervirulence is strongly linked to the overproduction of hypermucovisous capsule, but the underlining regulatory mechanism of hypermucoviscosity (HMV) has been elusive, especially at the post-transcriptional level mediated by small RNAs (sRNAs). Using a recently developed RNA interactome profiling approach, we have investigated the Hfq-associated sRNA regulatory network and established the first in vivo RNA-RNA interactome in HvKP. Our data reveal numerous interactions between sRNAs and HMV-related mRNAs, and identify a plethora of sRNA that inhibit or promote HMV. One of the strongest repressors of HMV was ArcZ, a conserved sRNA in the Enterobacteriaceae family. We found that ArcZ is activated by the master regulator of catabolite repression Crp, and down-regulates the expression of mlaA encoding an outer-membrane lipoprotein, leading to decreased HMV and virulence attenuation in mice. ArcZ significantly reduced HMV in several carbapenem-resistant and hypervirulent clinical isolates with diverse genetic background, suggesting it is an antisense RNA inhibitor of HMV with therapeutic potential. In summary, our work provides a comprehensive map of the RNA-RNA interaction network of HvKP and identifies ArcZ as a conserved repressor of HMV, providing novel insights into the mechanisms of posttranscriptional regulations of virulence.

2024-07-17 | GSE260738 | GEO

Project description:Transcriptomic data of edited CR-hvKP

| PRJNA1047885 | ENA

Preliminary investigation of iron acquisition mechanism in hypervirulent Klebsiella pneumoniae mediated by outer membrane vesicles

Project description:To investigate the role of outer membrane vesicles (OMVs) and related proteins in iron acquisition mechanism of hypervirulent Klebsiella pneumoniae (HVKP) and classic Klebsiella pneumoniae (cKP).

2021-08-21 | PXD028057 |

RNA interactome profiling in hypervirulent Klebsiella pneumoniae

Project description:To map the Hfq-mediated RNA regulatory network in HvKP, we harnessed the LiRIP-seq approach recently established in our lab, which enabled global profiling of RNA-RNA interactomes in live bacteria. Transformation of the pBAD-t4rnl1 plasmid into Klebsiella allowed us to express T4 RNA ligase 1 for proximity-ligation of RNAs in vivo, followed by co-immunoprecipitation (coIP) to enrich the Hfq-bound RNA transcripts (singleton reads) and ligated RNA fragments (chimeras: sRNA-mRNA, sRNA-sRNA, etc).

2024-07-17 | GSE243246 | GEO

Project description:draft genome sequence for the ST11 hvkp strains from China

| PRJNA391211 | ENA

Project description:Response of the gut microbiota to CR-Hvkp infection

| PRJNA974556 | ENA