Project description:LCMS/MS-file of the analysis of 286 extracts of 72 strains of the actinobacteria genus Planomonospora. Each strain was cultivated in 4 media, except for strains 82291 and 81182, which were only cultivated in media MC1, AF and AF2.
Also included are 4 media blanks (A3_E3_mod.mzXML, B3_F3_mod.mzXML, C3_G3_mod.mzXML, D3_H3_mod.mzXML). Total number of files: 290
Project description:Planomonospora sphaerica is a rare actinomycete that is a potential antibiotic producer. Here, we report the draft genome sequence of P. sphaerica strain JCM9374. This is the first genome report of a bacterium belonging to the genus Planomonospora The genome information of P. sphaerica will contribute to studies on the structure and function of antibiotics.
Project description:Despite an excellent track record, microbial drug discovery suffers from high rates of rediscovery. Better workflows for the rapid investigation of complex extracts are needed to increase throughput and to allow early prioritization of samples. In addition, systematic characterization of poorly explored strains is seldomly performed. Here, we report a metabolomic study of 72 isolates belonging to the rare actinomycete genus Planomonospora, using a workflow of commonly used open access tools to investigate its secondary metabolites. The results reveal a correlation of chemical diversity and strain phylogeny, with classes of metabolites exclusive to certain phylogroups. We were able to identify previously reported Planomonospora metabolites, including the ureylene-containing oligopeptide antipain, the thiopeptide siomycin including new congeners, and the ribosomally synthesized peptides sphaericin and lantibiotic 97518. In addition, we found that Planomonospora strains can produce the siderophore desferrioxamine or a salinichelin-like peptide. Analysis of the genomes of three newly sequenced strains led to the detection of 59 gene cluster families, of which three were connected to products found by LC-MS/MS profiling. This study demonstrates the value of metabolomic studies to investigate poorly explored taxa and provides a first picture of the biosynthetic capabilities of the genus Planomonospora.
Project description:LCMS/MS-file of the analysis of 286 extracts of 72 strains of the actinobacteria genus Planomonospora. Each strain was cultivated in 4 media, except for strains 82291 and 81182, which were only cultivated in media MC1, AF and AF2.
Also included are 4 media blanks (A3_E3_mod.mzXML, B3_F3_mod.mzXML, C3_G3_mod.mzXML, D3_H3_mod.mzXML). Total number of files: 290
