Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Xylella fastidiosa causes transcriptional shifts that precede tylose formation and starch depletion in xylem

ABSTRACT: Xylella fastidiosa causes transcriptional shifts that precede tylose formation and starch depletion in xylem

PROVIDER: PRJNA638536 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11973461.fastq.gz	Fastqsanger.gz
	SRR11973462.fastq.gz	Fastqsanger.gz
	SRR11973463.fastq.gz	Fastqsanger.gz
	SRR11973464.fastq.gz	Fastqsanger.gz
	SRR11973465.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Xylella fastidiosa causes transcriptional shifts that precede tylose formation and starch depletion in xylem

Project description:Pierce's disease (PD) in grapevine (Vitis vinifera) is caused by the bacterial pathogen Xylella fastidiosa. X. fastidiosa is limited to the xylem tissue and following infection induces extensive plant‐derived xylem blockages, primarily in the form of tyloses. Tylose‐mediated vessel occlusions are a hallmark of PD, particularly in susceptible V. vinifera. We temporally monitored tylose development over the course of the disease to link symptom severity to the level of tylose occlusion and the presence/absence of the bacterial pathogen at fine‐scale resolution. The majority of vessels containing tyloses were devoid of bacterial cells, indicating that direct, localized perception of X. fastidiosa was not a primary cause of tylose formation. In addition, we used X‐ray computed microtomography and machine‐learning to determine that X. fastidiosa induces significant starch depletion in xylem ray parenchyma cells. This suggests that a signalling mechanism emanating from the vessels colonized by bacteria enables a systemic response to X. fastidiosa infection. To understand the transcriptional changes underlying these phenotypes, we integrated global transcriptomics into the phenotypes we tracked over the disease spectrum. Differential gene expression analysis revealed that considerable transcriptomic reprogramming occurred during early PD before symptom appearance. Specifically, we determined that many genes associated with tylose formation (ethylene signalling and cell wall biogenesis) and drought stress were up‐regulated during both Phase I and Phase II of PD. On the contrary, several genes related to photosynthesis and carbon fixation were down‐regulated during both phases. These responses correlate with significant starch depletion observed in ray cells and tylose synthesis in vessels.

2021-01-19 | GSE152164 | GEO

Xylella fastidiosa causes transcriptional shifts that precede tylose formation and starch depletion in xylem.

Project description:Pierce's disease (PD) in grapevine (Vitis vinifera) is caused by the bacterial pathogen Xylella fastidiosa. X. fastidiosa is limited to the xylem tissue and following infection induces extensive plant-derived xylem blockages, primarily in the form of tyloses. Tylose-mediated vessel occlusions are a hallmark of PD, particularly in susceptible V. vinifera. We temporally monitored tylose development over the course of the disease to link symptom severity to the level of tylose occlusion and the presence/absence of the bacterial pathogen at fine-scale resolution. The majority of vessels containing tyloses were devoid of bacterial cells, indicating that direct, localized perception of X. fastidiosa was not a primary cause of tylose formation. In addition, we used X-ray computed microtomography and machine-learning to determine that X. fastidiosa induces significant starch depletion in xylem ray parenchyma cells. This suggests that a signalling mechanism emanating from the vessels colonized by bacteria enables a systemic response to X. fastidiosa infection. To understand the transcriptional changes underlying these phenotypes, we integrated global transcriptomics into the phenotypes we tracked over the disease spectrum. Differential gene expression analysis revealed that considerable transcriptomic reprogramming occurred during early PD before symptom appearance. Specifically, we determined that many genes associated with tylose formation (ethylene signalling and cell wall biogenesis) and drought stress were up-regulated during both Phase I and Phase II of PD. On the contrary, several genes related to photosynthesis and carbon fixation were down-regulated during both phases. These responses correlate with significant starch depletion observed in ray cells and tylose synthesis in vessels.

| S-EPMC7814960 | biostudies-literature

Heat Shock response of the phytopathogenic bacteria Xylella fastidiosa

Project description:This is the study of the Heat Shock response of phytopathogenic bacteria Xylella fastidiosa. This series keeps the 25 minutes 40oC stimulus response (Aug 2005). Keywords: stress response; heat shock response

2006-06-01 | GSE3044 | GEO

Xylella fastidiosa

Project description:Xylella fastidiosa Genome sequencing

| PRJNA574207 | ENA

Xylella fastidiosa

Project description:Xylella fastidiosa Genome sequencing- Iran

| PRJNA915469 | ENA

Xylella fastidiosa

Project description:Xylella fastidiosa Raw sequence reads

| PRJNA791935 | ENA

Expression analysis of Xylella fastidiosa PD Temecula strain grown in different media

Project description:Investigation of whole genome gene expression level changes in Xylella fastidiosa grown in minimal media XFM and XFM supplied with pectin or glucan (Host polysaccharides) , compared to cell grown in the complex media PWG. The cells grown in the minimal medium XFM supplied with host polysaccharides specially pectin are transmissible by the insect vector when delivered to the vector through artificial diet system. This does not happen with cells grown in the complex media. 4 (4 plex chips) study using total RNA recovered from 4 independents replicates for Xylella fastidiosa grown on PWG, XFM, XFM-glucan and XFM-pectin.

2009-10-03 | E-GEOD-18369 | biostudies-arrayexpress

Comparative genomic hybridization of Xylella fastidiosa 9a5c (pathogenic, reference strain) and J1a12 (non-pathogenic)

Project description:Comparison of genomic DNA from two strains of Xylella fastidiosa: 9a5c (pathogenic, reference strain) and J1a12 (non-pathogenic).

2004-04-01 | E-MEXP-22 | biostudies-arrayexpress

Xylella fastidiosa

Project description:Additional Xylella fastidiosa raw sequence reads

| PRJNA795489 | ENA

Xylella fastidiosa

Project description:RpfF-dependent regulon of Xylella fastidiosa

| PRJNA167936 | ENA