Project description:Expression profiling of treatment of E. histolytica HM-1:IMSS trophozoites with 5-Aza cytidine, an inhibitor of DNA methyltransferase Keywords: drug treatment HM1 trophozoites were treated for 3 or 7 days with 23 micromolar 5-Aza cytidine, and RNA extracted and analyzed on a custom Affymetrix array. Data from these two arrays is to be compared to expression data from HM1 trophozoites grown without drug exposure.
Project description:A myb domain containing protein of the SHAQKY family, EhMyb-dr, was overexpressed in E. histolytica trophozoites, and effects on transcription were measured. Two arrays of trophozoites expressing Myc tagged EhMyb-dr were compared to two arrays performed on wild type trophozoites. The effects of transfection and drug were controlled for by including data from trophozoites expressing irrelevant plasmids, and grown in the same level of G418. All parasites were of strain HM-1:IMSS
Project description:Expression profiling of treatment of E. histolytica HM-1:IMSS trophozoites with 5-Aza cytidine, an inhibitor of DNA methyltransferase Keywords: drug treatment