Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Evaluation of Integrin aE-positive and negative cells

ABSTRACT: Evaluation of Integrin aE-positive and negative cells

PROVIDER: PRJNA638956 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Evaluation of Integrin aE-positive and negative cells

Project description:Etrolizumab, a humanized monoclonal antibody that selectively binds the b7 subunit of the heterodimeric integrins a4b7 and aEb7, is currently in development for patients with moderate-to-severely active UC. Integrin aE forms a heterodimer with b7; and aEb7 interacts with E-cadherin to mediate retention of aEb7+ cells in the intestinal epithelium. Baseline integrin aE levels have been identified as a potential predictive biomarker for etrolizumab response. Additional markers, including granzyme A, have also been identified and are being developed in partnership with Roche Molecular as potential predictive biomarkers for etrolizumab along with integrin aE. Extensive work has been done to characterize gut aE+ T cells by immunohistochemistry, flow cytometry and qPCR. We hope to use this project to validate differences between aE+ and aE- cell populations using CD4, CD8 and granzyme A expression as controls. The results from this project will be used to pilot single cell sequencing of CD3+aE+ cells isolated from the gut mucosa to identify cellular heterogeneity within aE+ cells under normal and inflammatory conditions. Materials and methods: Mucosal lymphocytes were isolated using a collagenase digestion from surgical specimens taken during routine gastrointestinal resections of diverticulitis and ulcerative colitis patients. CD45+TCRab+TCRgd- cells were sorted into aE+ and aE- groups.

2021-07-23 | GSE152320 | GEO

Ae. aegypti and Ae. albopictus

Project description:The mosquito vectors Ae. aegypti and Ae. albopictus Targeted loci

| PRJNA523634 | ENA

HIV positive (n=4) and HIV negative (n=4) serum miRNA screen

Project description:Evaluation of all human miRNAs in HIV positive and negative individuals. Used to validate RNA extraction from serum. Also used to determine which miRNAs were present in the serum of HIV+ individuals.

2017-03-28 | GSE97108 | GEO

Hippocampal cells multiplex positive and negative engrams

Project description:Hippocampal cells multiplex positive and negative engrams

| PRJNA816561 | ENA

Hippocampal cells multiplex positive and negative engrams

Project description:Hippocampal cells multiplex positive and negative engrams

| PRJNA858461 | ENA

Transcriptome of Chat positive and negative ILC2s

Project description:Transcriptome of Chat positive and negative ILC2s

| PRJNA691600 | ENA

TAF1 is critical for AE driven leukemogenesis [RNA-seq]

Project description:TAF1 is essential for AE driven leukemogenesis. Depletion of TAF1 impairs the recruitment of AE to its target genes, interfering with its control of gene expression. The bromodomains of TAF1 associate with K43 acetylated AE and this association plays a role in the proliferation of AE expressing AML cells.

2019-09-11 | GSE115121 | GEO

TAF1 is critical for AE driven leukemogenesis [ChIP-seq]

2019-09-11 | GSE115115 | GEO

TAF1 is critical for AE driven leukemogenesis [ATAC-seq]

Project description:TAF1 is essential for AE driven leukemogenesis. Depletion of TAF1 impairs the recruitment of AE to its target genes, interfereing with its control of gene expression. The bromodomains of TAF1 associate with K43 acetylated AE and this association plays a role in the proliferationof AE expressing AML cells.

2019-09-11 | GSE134018 | GEO

Transcription profiling of mouse epidermal cells sorted into three populations based on alpha 6 integrin, CD34 or Sca-1 surface marker expression

Project description:Epidermal keratinocytes were isolated from adult murine skin and FACS sorted into three populations based on their surface maker expression of alpha 6 integrin, CD34 and Sca-1. <br>- Alpha 6 positive cells constitute the entire population of basal or proliferative keratinocytes from the interfollicular epidermis and hair follicle. Both BIB and Bulge cells are contained within the total population of alpha 6 positive cells. <br>- BIB cells reside in the hair follicle in a region between the infundibulum and bulge and these cells are characterized by low alpha 6 integrin surface levels and are negative for CD34 and Sca-1 surface proteins. <br>- Bulge cells are derived from the bulge region of the hair follicle and are characterized by low + high alpha 6 integrin surface levels and are positive for CD34 but negative for Sca-1.

2009-11-16 | E-MEXP-928 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data