Gene expression profile of TPT1 gene knock downed human ES-NSC
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ABSTRACT:
PROVIDER: PRJNA639041 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12002117.fastq.gz | Fastqsanger.gz | |||
| SRR12002118.fastq.gz | Fastqsanger.gz |
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Project description:To evaluate the function of TPT1 in human ES-derived neural stem cells (ES-NSCs), we knock downed TPT1 gene expression based on the lentivirus system expressing shTPT1 RNA with control. We isolated RNA from ES-NSCs 2 days after infection and subjected to RNA-seq analysis. In silico data analysis supported the function of TPT1 related to cell cycle regulation, consistent with in vitro experimental results.
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Project description:Pluripotent stem cells have been shown to have unique nuclear properties, e.g., hyperdynamic chromatin and large, condensed nucleoli. However, the contribution of the latter unique nucleolar character to pluripotency has not been well understood. Here, we show fibrillarin (FBL), a critical methyltransferase for ribosomal RNA (rRNA) processing in nucleoli, as one of the proteins highly expressed in pluripotent embryonic stem (ES) cells. Stable expression of FBL in ES cells prolonged the pluripotent state of mouse ES cells cultured in the absence of leukemia inhibitory factor (LIF). Analyses using deletion mutants and a point mutant revealed that the methyltransferase activity of FBL regulates stem cell pluripotency. Knock down of this gene led to significant delays in rRNA processing, growth inhibition, and apoptosis in mouse ES cells. Interestingly, both partial knock down of FBL and treatment with actinomycin D, an inhibitor for rRNA synthesis, induced the expression of differentiation markers in the presence of LIF and promoted stem cell differentiation into neuronal lineages. Moreover, we identified p53 signaling as the regulatory pathway for pluripotency and differentiation of ES cells. These results suggest that proper activity of rRNA production in nucleoli is a novel factor for the regulation of pluripotency and differentiation ability of ES cells.
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