Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Eastern Newt

ABSTRACT: 16S bacterial communities within the gut of the eastern newt Targeted loci environmental

PROVIDER: PRJNA639116 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12004800_1.fastq.gz	Fastqsanger.gz
	SRR12004800_2.fastq.gz	Fastqsanger.gz
	SRR12004801_1.fastq.gz	Fastqsanger.gz
	SRR12004801_2.fastq.gz	Fastqsanger.gz
	SRR12004802_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 70

Similar Datasets

Microarray Analysis of microRNA Expression during Notophthalmus viridescens (red-spotted newt) heart regeneration

Project description:The adult vertebrate red spotted newt is a champion of regeneration, demonstrating an amazing ability to regenerate damaged organs and tissues back to an uninjured state without the formation of scar or reduction in function. By developing a novel cardiac resection strategy, our group recently demonstrated that newt hearts could morphologically and functionally regenerate, without scarring, within a period of 2-3 months following injury. MicroRNAs (miRs) have been widely publicized as essential post-transcriptional gene regulators in a variety of biological processes, including regeneration. We have conducted a microarray screen for vertebrate miRs, with several candidate miRs showing significant differential expression at important time-points following injury to the newt heart. The newt microRNA expression between uninjured hearts and regenerating hearts, 7 and 21 days post-injury (dpi), was compared by microarray analysis. Three paired samples were analyzed: Uninjured, 7dpi and 21dpi newt hearts. Three arrays were hybridized comparing two-paired samples each time.

2013-09-24 | E-GEOD-44547 | biostudies-arrayexpress

Microarray Analysis of microRNA Expression during Notophthalmus viridescens (red-spotted newt) heart regeneration

2013-09-24 | GSE44547 | GEO

Archival chromatin sequencing in eastern water dragon museum specimens

Project description:Formalin induces inter- and intra-molecular crosslinks within exposed cells. This cross-linking can be exploited to characterise chromatin state as in the MNase (micrococcal nuclease) assays. Our team aims to optimise these assays for application in museum preserved formalin-exposed specimens. To do so, we applied an optimised MNase assay to fresh-frozen and archival eastern water dragon specimens, as old as 1905. We found that heavy formalin fixation modulates rather than eliminates signatures of differential chromatin accessibility and that these chromatin profiles are sex-specific and environmental condition dependent.

2024-03-01 | GSE256157 | GEO

Macrophages modulate fibrosis during newt lens regeneration [Nvir]

Project description:Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Here we generated a transgenic newt reporter line in which macrophages can be visualized in vivo. Using this new tool, we analyzed the location of macrophages during lens regeneration. We uncovered early gene expression changes using bulk RNAseq in two newt species, Notophthalmus viridescens and Pleurodeles waltl. Next, we used clodronate liposomes to deplete macrophages, which inhibited lens regeneration in both newt species. Macrophage depletion induced the formation of scar-like tissue, an increased and sustained inflammatory response, an early decrease in iris pigment epithelial cell (iPEC) proliferation and a late increase in apoptosis. Some of these phenotypes persisted for at least 100 days and could be rescued by exogenous FGF2. Re-injury alleviated the effects of macrophage depletion and re-started the regeneration process. Together, our findings highlight the importance of macrophages in facilitating a pro-regenerative environment in the newt eye, helping to resolve fibrosis, modulating the overall inflammatory landscape and maintaining the proper balance of early proliferation and late apoptosis.

2024-05-22 | GSE236907 | GEO

Macrophages modulate fibrosis during newt lens regeneration [PWaltl]

2024-05-22 | GSE236906 | GEO

MicroRNA sequencing and analysis of the newt heart

Project description:MicroRNA sequencing using Ion Torrent Proton platform of the undamaged heart of the red spotted newt Notophthalmus viridescens. MicroRNAS were identified using MIRPIPE

2021-05-24 | GSE69537 | GEO

Comparative transcriptome analysis of transgenic Drosophila misexpressing novel Notophthalmus viridescens (newt) genes

Project description:To obtain a quick glimpse into possible function of five novel Notopthalmus viridescens (newt) genes (and given certain restrains with transgenic newts, such as time) we express these genes using transgenic Drosophila melanogaster. We generated the transgenic flies containing newt candidate genes, and prepared samples for RNA sequencing to evaluate the overall role of candidate genes in tissue development and layout map for future studies.

2021-11-03 | GSE134348 | GEO

marine metagenome

Project description:bacterial community in mesocosms targeted loci (16S rRNA gene) environmental

| PRJNA307158 | ENA

Bacteria

Project description:Bacterial community 16S Targeted loci

| PRJNA386165 | ENA

Forest bacteria in eastern China

Project description:forest bacterial diversity in eastern China

| PRJNA420924 | ENA