Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Small non-coding RNA sequencing of human monocyte-derived macrophages

ABSTRACT: Small non-coding RNA sequencing of human monocyte-derived macrophages

PROVIDER: PRJNA639121 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12004572.fastq.gz	Fastqsanger.gz
	SRR12004573.fastq.gz	Fastqsanger.gz
	SRR12004574.fastq.gz	Fastqsanger.gz
	SRR12004575.fastq.gz	Fastqsanger.gz
	SRR12004576.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Small non-coding RNA sequencing of human monocyte-derived macrophages

Project description:The goal of this experiment was to identify the differential expression of microRNAs in human polarised macrophages. We used monocyte-derived macrophages isolated from 8 different healthy invididuals. Differentiated macrophages were polarised towards the pro-inflammatory M1-like phenotype, by using LPS and INF- . Total RNA was extracted and sent for small-RNA sequencing to the company Novogene.

2021-12-01 | GSE152371 | GEO

Monocyte-derived Macrophages

Project description:Monocyte-derived Macrophages

| PRJNA948022 | ENA

cART Treated monocyte derived macrophages

Project description:cART Treated monocyte derived macrophages

| PRJNA801872 | ENA

Transcriptome profile of human dermal macrophages and monocyte-derived macrophages

Project description:DC, monocyte and macrophage networks are evolutionarily conserved but the distinct subsets have been difficult to distinguish due to shared overlapping phenotypic markers between the cells. Using transcriptome microarray profiling of human and mouse mononuclear phagocyte subsets, we have distinguished human dermal DCs from monocyte-derived cells and macrophages. Gene Expression from total RNA from human dermal macrophages, epidermal LCs and CD14+ cells subsets purified by FACS

2014-10-15 | E-GEOD-60317 | biostudies-arrayexpress

Expression data from monocytes and monocyte derived macrophages

Project description:Macrophages play a key role in both innate and adaptive immunity, but our knowledge on the changes in transcription regulation that occurs during their differentiation from monocytes is still limited. In this study, we used a meta-analysis followed by a systems biology approach for the identification of differentially expressed genes between monocytes and macrophages and possible regulators of these changes in transcription. Based on the pattern of gene expression change, transcription regulator analysis predicted a decrease in Enhancer of Zeste homolog 2 (EZH2), a histone 3 lysine 27 methyl transferase, activity after differentiation of monocytes into macrophages. This inhibition was validated by a significant decrease in trimethylated H3K27 during differentiation of both human primary monocytes into macrophages and the THP-1 cell line into macrophage-like cells. Overexpressing EZH2 during differentiation of monocytes and THP-1 cells obstructs cellular adhesion, thus preventing the first step in differentiation. Another facet of macrophage differentiation is the cessation of proliferation, and inhibition of EZH2 by the small molecule inhibitor GSK126 in THP-1 cells indeed impedes proliferation. This study shows an important part for epigenetic changes during monocyte differentiation. It highlights the role of EZH2 activity behind the changes needed in adhesion and proliferation mechanisms for macrophage formation. Monocytes isolated from human peripherial mononuclear cells were differentiated in monocyte derived macrophages by M-CSF stimulation

2013-11-22 | E-GEOD-52647 | biostudies-arrayexpress

cART Treated monocyte derived macrophages

Project description:primary human monocyte derived macrophages (MDMs) treated with antiretrovirals, specifically Atripla (A) or Triumeq (T)

2022-02-28 | GSE195708 | GEO

Transcription profiling of human monocyte-derived macrophages treated with IFN alpha

Project description:To study effects of IFNalpha treatment on monocyte-derived macrophages which may influence susceptibility or resistance to HIV. Experiment Overall Design: Human monocyte-derived macrophages were stimulated with IFNalpha and analyzed for global gene expression.

2009-07-05 | E-GEOD-16755 | biostudies-arrayexpress

PPARβ/δ ligand response in monocyte derived macrophages

Project description:The PPARD ligand response (agonist and inverse agonist) in monocyte derived macrophages from 3 healthy donors was assesed via RNAseq.

2016-05-13 | E-MTAB-3398 | biostudies-arrayexpress

Bovine monocyte derived macrophages from peripheral blood

Project description:Bovine monocyte derived macrophages from peripheral blood

| PRJNA620358 | ENA

Tumor cell efferocytosis in monocyte derived macrophages

Project description:Tumor cell efferocytosis in monocyte derived macrophages

| PRJNA763980 | ENA