Project description:To study the underlying mechanism of erinacines derived from Hericium erinaceus in neuroprotective effect against neurodegenerative diseases, we used the next-generation sequencing technology and bioinformatic analyses, erinacine S was found to cause the accumulation of neurosteroids in neurons. Additionally, we found that erinacine S enhances neurite outgrowth in a cell autonomous fashion of primary neurons. It also promotes post-injury axon regeneration of PNS neurons and enhances regeneration on inhibitory substrates of CNS neurons. This research uncovers a previously unknown effect of erinacine S on promoting neuronal regeneration via raising the level of neurosteroids.
Project description:The genome of H. erinaceus original strain HEA was reported 38.16 Mb, encoding 9,780 predicted genes by single-molecule, real-time sequencing technology. The phylogenomic analysis showed that H. erinaceus had the closest evolutionary affinity with Dentipellis sp. The polysaccharide content in the fermented mycelia of mutated strains HEB and HEC, which obtained by ARTP mutagenesis in our previous study, was improved by 23.25% and 47.45%, and a new β-glucan fraction with molecular weight 1.056×10^6 Da was produced in HEC. Integrative analysis of transcriptome and proteomics showed the upregulation of the carbohydrate metabolism pathway modules in HEB and HEC might lead to the increased production of glucose-6P and promote the repeating units synthesis of polysaccharides. qPCR and PRM analysis confirmed that most of the co-enriched and differentially co-expressed genes involved in carbohydrate metabolism shared a similar expression trend with the transcriptome and proteome data in HEB and HEC. Heatmap analysis showed a noticeably decreased protein expression profile of the RAS-cAMP-PKA pathway in HEC with a highly increased 47.45% of polysaccharide content. The S phase progression blocking experiment further verified that the RAS-cAMP-PKA pathway's dysfunction might promote high polysaccharide and β-glucan production in the mutant strain HEC.
