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Improved maturity and yield of pluripotent stem cell-derived hepatocytes for 2D and 3D modeling of human liver pathophysiology

ABSTRACT: Improved maturity and yield of pluripotent stem cell-derived hepatocytes for 2D and 3D modeling of human liver pathophysiology

PROVIDER: PRJNA639504 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12015807.fastq.gz	Fastqsanger.gz
	SRR12015808.fastq.gz	Fastqsanger.gz
	SRR12015809.fastq.gz	Fastqsanger.gz
	SRR12015810.fastq.gz	Fastqsanger.gz
	SRR12015811.fastq.gz	Fastqsanger.gz

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Improved maturity and yield of pluripotent stem cell-derived hepatocytes for 2D and 3D modeling of human liver pathophysiology

Project description:Robust and scalable differentiation of human pluripotent stem cells into high-quality hepatocyte-like cells (HLC) is achieved by acting on Wnt/b-catenin and TGFb pathways and taking advantage of latest knowledge on human liver development. Obtained HLC are comparable to primary human hepatocytes and superior to stem cell-derived hepatocytes generated with previously described protocols. As a proof of concept, such HLC were used to assess drug hepatotoxicity and study human ductal plate and bile duct morphogenesis in a complex liver organoid model.

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Project description:The contradiction between ‘high-yielding’ and ‘early-maturing’ hampers further improvement on annual rice yield. Here we reported the positional cloning of a major maturity duration-regulatory gene, Early flowering-completely dominant (Ef-cd), and demonstrated that natural variation in Ef-cd could be used to overcome the above contradictory. The Ef-cd locus gives rise to a long noncoding RNA (lncRNA) antisense transcript overlapping the OsSOC1 gene. Ef-cd lncRNA expression positively correlates with the expression of OsSOC1 and H3K36me3 deposition. Field test comparisons of early-maturing Ef-cd near-isogenic lines (NILs) with their wild types as well as the derivative early-maturing hybrids with their wild-type hybrids conducted under different latitudes determined that early-maturing Ef-cd allele shortens maturity duration (ranging from 7 to 20 days) without a concomitant yield penalty. Ef-cd facilitates nitrogen utilization and also improves the photosynthesis rate. Analysis of 1,439 elite hybrid rice varieties revealed that the 16 homozygotes and 299 heterozygotes possessing Ef-cd matured significantly earlier. Therefore, Ef-cd could be a vital contributor of elite early-maturing hybrid varieties in balancing grain yield with maturity duration.

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