Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mechanics of a multilayer epithelium instruct tumor architecture and function [E15]

ABSTRACT: Mechanics of a multilayer epithelium instruct tumor architecture and function [E15]

PROVIDER: PRJNA639528 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12016179_1.fastq.gz	Fastqsanger.gz
	SRR12016179_2.fastq.gz	Fastqsanger.gz
	SRR12016180_1.fastq.gz	Fastqsanger.gz
	SRR12016180_2.fastq.gz	Fastqsanger.gz
	SRR12016181_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Mechanics of a multilayer epithelium instruct tumor architecture and function

Project description:Mechanics of a multilayer epithelium instruct tumor architecture and function

| PRJNA639524 | ENA

Mechanics of a multilayer epithelium instruct tumor architecture and function [adult]

Project description:Mechanics of a multilayer epithelium instruct tumor architecture and function [adult]

| PRJNA639529 | ENA

Mechanics of a multilayer epithelium instruct tumor architecture and function [E15]

Project description:Loss of normal tissue architecture is a hallmark of oncogenic transformation. Mechanical forces sculpt tissue architectures during morphogenesis. However, their origins and consequences during tumorigenesis remain elusive. In skin, premalignant basal cell carcinomas (BCCs) form ‘buds’, while invasive squamous cell carcinomas (SCC) initiate as ‘folds’. Using computational modeling, genetic manipulations and biophysical measurements, we identify the biophysical underpinnings and biological consequences of these tumor architectures. Cell proliferation and actomyosin contractility dominate tissue architectures in monolayer, but not multilayer epithelia. In stratified epidermis, softening and enhanced remodeling of the basement membrane (BM) promote tumor budding, while BM stiffening promotes folding. We show that additional key forces stem from progenitor cell stratification and differentiation Tumor-specific suprabasal stiffness gradients are generated as oncogenic lesions progress toward malignancy, which we computationally predict alter extensile tensions on the tumor BM. The pathophysiologic ramifications of this prediction are profound. Genetically decreasing BM stiffness elevates BM tensions in silico and potentiates invasive SCC progression in vivo. Our findings suggest that mechanical forces, exerted from above and below progenitors of multilayered epithelia, are integrally linked and function to shape premalignant tumor architectures and influence tumor progression.

2020-07-01 | GSE152486 | GEO

Mechanics of a multilayer epithelium instruct tumor architecture and function [adult]

2020-07-01 | GSE152487 | GEO

Mechanics of a multilayer epithelium instruct tumour architecture and function.

Project description:Loss of normal tissue architecture is a hallmark of oncogenic transformation1. In developing organisms, tissues architectures are sculpted by mechanical forces during morphogenesis2. However, the origins and consequences of tissue architecture during tumorigenesis remain elusive. In skin, premalignant basal cell carcinomas form 'buds', while invasive squamous cell carcinomas initiate as 'folds'. Here, using computational modelling, genetic manipulations and biophysical measurements, we identify the biophysical underpinnings and biological consequences of these tumour architectures. Cell proliferation and actomyosin contractility dominate tissue architectures in monolayer, but not multilayer, epithelia. In stratified epidermis, meanwhile, softening and enhanced remodelling of the basement membrane promote tumour budding, while stiffening of the basement membrane promotes folding. Additional key forces stem from the stratification and differentiation of progenitor cells. Tumour-specific suprabasal stiffness gradients are generated as oncogenic lesions progress towards malignancy, which we computationally predict will alter extensile tensions on the tumour basement membrane. The pathophysiologic ramifications of this prediction are profound. Genetically decreasing the stiffness of basement membranes increases membrane tensions in silico and potentiates the progression of invasive squamous cell carcinomas in vivo. Our findings suggest that mechanical forces-exerted from above and below progenitors of multilayered epithelia-function to shape premalignant tumour architectures and influence tumour progression.

| S-EPMC7787055 | biostudies-literature

Mechanics of a multilayer epithelium instruct tumor architecture and function

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-07-01 | GSE152488 | GEO

YAP regulates cell mechanics by controlling focal adhesion assembly

Project description:Hippo effectors YAP/TAZ act as on-off mechanosensing switches by sensing modifications in extracellular matrix (ECM) composition and mechanics. The regulation of their activity has been described so far through a hierarchical model in which elements of Hippo pathway are under the control of Focal Adhesions (FAs). Here we unveiled the molecular mechanism by which cell spreading and RhoA GTPase control FA formation through YAP to stabilize the anchorage of actin cytoskeleton to cell membrane. This mechanism required YAP co-transcriptional function and involved the activation of genes encoding for integrins and FA docking proteins. Tuning YAP transcriptional activity led to the modification of cell mechanics, force development, adhesion strength, determined cell shaping, migration and differentiation. These results provide new insights into the mechanism of YAP mechanosensing activity and qualify Hippo effector as the key determinant of cell mechanics in response to ECM cues.

2017-05-17 | E-MTAB-5217 | biostudies-arrayexpress

Rosa chinensis strain:E15

Project description:Rosa chinensis strain:E15 Map

| PRJNA72593 | ENA

Marinimicrobia bacterium JGI 0000039-E15

Project description:SAR406 cluster bacterium JGI 0000039-E15

| PRJNA192292 | ENA

Lactiplantibacillus plantarum strain:VHProbi E15

Project description:Lactiplantibacillus plantarum VHProbi E15 genome sequencing

| PRJNA821998 | ENA