Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pulmonary effects of inhaled diesel particles in rats

ABSTRACT: Pulmonary effects of inhaled diesel particles in rats

PROVIDER: PRJNA640183 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12041800_1.fastq.gz	Fastqsanger.gz
	SRR12041800_2.fastq.gz	Fastqsanger.gz
	SRR12041801_1.fastq.gz	Fastqsanger.gz
	SRR12041801_2.fastq.gz	Fastqsanger.gz
	SRR12041802_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 144

Similar Datasets

Pulmonary effects of inhaled diesel particles in rats

Project description:The pulmonary response to inhalation exposure to diesel exhaust particles (DEP) was investigated in a rat model. Adult male Sprague-Dawley rats were exposed by whole-body inhalation to air or an aerosol containing DEP at concentrations of 200 or 1000 mg/m3, 6 hours/day for 4 days. The control and DEP-exposed rats were euthanized at post-exposure time intervals of 1, 7, or 27 days and pulmonary inflammatory, cytotoxic and oxidant responses were determined. Analysis of bronchoalveolar lavage parameters of toxicity such as lactate dehydrogenase activity, oxidant generation, and inflammation did not reveal any significant pulmonary toxicity in the DEP-exposed rats. The lung gene expression profiles did not change significantly in the DEP exposed rats compared with the controls. The data obtained from the present study demonstrated that DEP inhalation exposure under the conditions employed in the present study did not result in any significant lung toxicity in the rats.

2022-12-31 | GSE152720 | GEO

Pulmonary effects of inhaled diesel exhaust and fracking sand particles in rats

Project description:Pulmonary effects of inhaled diesel exhaust and fracking sand particles in rats

| PRJNA1011879 | ENA

Pulmonary effects of inhaled diesel exhaust and fracking sand particles in rats

Project description:The pulmonary response to inhalation exposure to diesel exhaust particles (DEP) and fracking sand particles (FSD) was investigated in a rat model. Healthy adult male Sprague-Dawley rats were exposed by whole-body inhalation to air (control) or an aerosol containing DEP at a concentration of 1.0 mg/m3 combined with FSD at concentrations of 30 mg/m3, 6 hours/day for 4 days. The control and DEP/FSD exposed rats were euthanized at post-exposure time intervals of 1, 7, or 27 days and pulmonary inflammatory, cytotoxic, and oxidant responses were determined. Analysis of bronchoalveolar lavage parameters of toxicity such as lactate dehydrogenase activity, oxidant generation, and inflammation revealed only minimal changes in pulmonary toxicity in the DEP/FSD-exposed rats, compared with the time-matched controls. The lung gene expression profiles showed only minimal changes in the DEP/FSD exposed rats compared with the controls. Specifically, there were a total of 6 genes significantly differentially expressed (fold change >1.5 and FDR p<0.05) only at the one-day post-exposure group. The data obtained from the present study demonstrated that DEP/FSD inhalation exposure, under the conditions employed in the present study, resulted in only minimal changes in lung toxicity and gene expression profile in the rats.

2024-12-01 | GSE242155 | GEO

DNA methylation changes in A549 cells after four weeks of exposure to 30 μg/ml diesel exhaust particles.

Project description:Diesel exhaust particles (DEP), which contain hazardous compounds, are emitted during the combustion of diesel. As approximately one-third of the vehicles worldwide use diesel, there are growing concerns on the risks posed by DEP to human health. Long-term exposure to DEP is associated with airway hyperresponsiveness, pulmonary fibrosis, and inflammation; however, the molecular mechanisms behind the effects of DEP on the respiratory tract are poorly understood. Such mechanisms can be addressed by examining transcriptional and DNA methylation changes. In this study, we investigated the effect of 4 weeks exposure to 30 μg/ml DEP on DNA methylation levels in A549 cells.

2024-08-05 | GSE267215 | GEO

Lung cell responses to ship diesel exhaust particles

2015-02-01 | GSE63962 | GEO

Transcriptional changes in A549 cells after four weeks of exposure to 30 μg/ml diesel exhaust particles measured by RNA-seq.

2024-08-05 | GSE267213 | GEO

Effect of Diesel Exhaust Particles in Normal Human Embryonic Lung Cell (WI-38) Line

Project description:We performed RNA sequencing to identify genes and pathways induced by Diesel exhaust particles exposure in WI-38 cells.

2021-02-14 | GSE166729 | GEO

Identification of a dose of diesel exhaust particles that is sufficient to induce significant DNA methylation changes in A549 cells after four weeks of exposure.

Project description:Diesel exhaust particles (DEP), which contain hazardous compounds, are emitted during the combustion of diesel. As approximately one-third of the vehicles worldwide use diesel, there are growing concerns on the risks posed by DEP to human health. Long-term exposure to DEP is associated with airway hyperresponsiveness, pulmonary fibrosis, and inflammation; however, the molecular mechanisms behind the effects of DEP on the respiratory tract are poorly understood. Such mechanisms can be addressed by examining transcriptional and DNA methylation changes. In this study, we investigated DEP dose sufficient to induce significant DNA methylation changes after four weeks of exposure.

2024-08-05 | GSE267214 | GEO

Lung cell responses to ship diesel exhaust particles

Project description:Human BEAS-2B bronchial epithelial cells were exposed directly at the air-liquid interphase towards exhaust gas and particles of a ship engine. The goal was to compare the responses towards different fuel combustions. The engine run either on diesel fuel (DF) or on Heavy Fuel Oil (HFO). The lung cells were exposed 3 times to each combustion aerosol (DF or HFO). The duration of the exposure was 4h. The cells were seeded into transwell-inserts 24h before exposure. Within each exposure 3 transwell-inserts were exposed to the complete aerosol and 3 transwell-inserts were exposed to the filtered aerosol. Effects of the complete aerosol were referenced against the filtered aerosol to determine the effects of the aerosol particles.

2015-02-01 | E-GEOD-63962 | biostudies-arrayexpress

Transcriptional changes in A549 cells after four weeks of exposure to 30 μg/ml diesel exhaust particles measured by Agilent SurePrint G3 Human Gene Expression Microarray.

2024-08-05 | GSE267216 | GEO