Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Multiomics profiling of young and old quiescent skeletal muscle stem cells [aging WGBS]

ABSTRACT: Multiomics profiling of young and old quiescent skeletal muscle stem cells [aging WGBS]

PROVIDER: PRJNA640418 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Multiomics profiling of young and old quiescent skeletal muscle stem cells [aging WGBS]

Project description:To uncover new pathways that are important for skeletal muscle stem cell aging, we performed multiomics profiling, including transcriptomics, DNA methylomics, proteomics, and metabolomics on quiescent muscle stem cells from young and old mice. Our goals were to discover pathways that have been overlooked by isolated profiling approaches and to gain insight into which changes are causal, compensatory, correlational, and consequential. In our work, we found that glutathione metabolism is a key pathway of muscle stem cell aging that involves a compensatory feedback loop. Follow-up experiments showed that old muscle stem cells actually form a dichotomy between glutathione-high muscle stem cells and glutathione-low muscle stem cells. RNA-Seq showed that glutathione-high old muscle stem cells are able to synthesize adequate glutathione and thus compensate adequately for oxidative stress with increased glutathione turnover, while glutathione-low old muscle stem cells have failed to compensate for oxidative stress metabolically and instead show increased inflammatory signaling.

2023-03-20 | GSE152797 | GEO

Multiomics profiling of young and old quiescent skeletal muscle stem cells

Project description:Multiomics profiling of young and old quiescent skeletal muscle stem cells

| PRJNA640416 | ENA

Multiomics profiling of young and old quiescent skeletal muscle stem cells [GSH RNA-seq]

Project description:To uncover new pathways that are important for skeletal muscle stem cell (MuSC) aging, we performed multiomics profiling, including transcriptomics, DNA methylomics, proteomics, and metabolomics on quiescent MuSCs from young and old mice. Our goals were to discover pathways that have been overlooked by isolated profiling approaches and to gain insight into which changes are causal, compensatory, correlational, and consequential. In our work, we found that glutathione metabolism is a key pathway of MuSC that involves a compensatory feedback loop. Follow-up experiments showed that old MuSCs actually form a dichotomy between glutathione-high MuSCs and glutathione-low MuSCs. RNA-Seq showed that glutathione-high old MuSCs are able to synthesize adequate glutathione and thus compensate adequately for oxidative stress with increased glutathione turnover, while glutathione-low old MuSCs have failed to compensate for oxidative stress metabolically and instead show increased inflammatory signaling.

2023-03-20 | GSE152796 | GEO

Multiomics profiling of young and old quiescent skeletal muscle stem cells [aging RNA-seq]

2023-03-20 | GSE152793 | GEO

Multiomics profiling of young and old quiescent skeletal muscle stem cells [aging RNA-seq]

Project description:Multiomics profiling of young and old quiescent skeletal muscle stem cells [aging RNA-seq]

| PRJNA640409 | ENA

Multiomics profiling of young and old quiescent skeletal muscle stem cells [GSH RNA-seq]

Project description:Multiomics profiling of young and old quiescent skeletal muscle stem cells [GSH RNA-seq]

| PRJNA640417 | ENA

Multiomics profiling of young and old quiescent skeletal muscle stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-20 | GSE152798 | GEO

RNA-seq of young and quiescent MRC-5 human fibroblasts

Project description:Quiescent MRC-5 fibroblasts were compared to young fibroblasts 6 samples: 3 biological replicates for each age group: young and quiescent MRC-5 cells. 50bp, single-end reads, no strand-specific reads

2014-11-06 | E-GEOD-60883 | biostudies-arrayexpress

RNA-seq of young and quiescent MRC-5 human fibroblasts

Project description:Quiescent MRC-5 fibroblasts were compared to young fibroblasts Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)

2014-11-06 | GSE60883 | GEO

RNA-seq of young and quiescent (9 days) MRC-5 human fibroblasts

Project description:Comparison of gene expression profiles of quiescent MRC-5 fibroblasts and young MRC-5 fibroblasts. Jena Centre for Systems Biology of Ageing – JenAge (www.jenage.de)

2021-10-19 | GSE118693 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data