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RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic and fructo-oligosaccharides

ABSTRACT: RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic and fructo-oligosaccharides

PROVIDER: PRJNA640685 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12057821.fastq.gz	Fastqsanger.gz
	SRR12057822.fastq.gz	Fastqsanger.gz
	SRR12057823.fastq.gz	Fastqsanger.gz
	SRR12057824.fastq.gz	Fastqsanger.gz
	SRR12057825.fastq.gz	Fastqsanger.gz

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RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic and fructo-oligosaccharides

Project description:Purpose:Our data significantly advance understanding of Probiotics and Fructooligosaccharide regulatory mechanism in ileum of Taiping chicken Methods: Using RNA-seq analysis to study the gene expression in ileum tissue after Taiping chicken was given Probiotics and Fructooligosaccharide Results: A total of 164,629,826 and 149,883,266 raw reads were generated from the CT and MP_ FOS, respectively . After removing the interference data, about 164,028,872 and 149,364,852 clean reads were obtained Conclusions: through high-throughput sequencing of the six libraries from ileum of Taiping chicken, the expression level of mRNA has significant changes after given probiotics

2023-06-16 | GSE152878 | GEO

RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic

Project description:Purpose:Our data significantly advance understanding of probiotic regulatory mechanism of miRNA in ileum of Taiping chicken Methods: Using RNA-seq analysis to study the gene expression in ileum tissue after Taiping chicken was given probiotics Results: A total of 164,629,826 and 156,180,764 raw reads were generated from the CT and MP, respectively. After removing the interference data, about 164,028,872 and 155,579,470 clean reads were obtained Conclusions: through high-throughput sequencing of the six libraries from ileum of Taiping chicken, the expression level of mRNA has significant changes after given probiotics

2023-05-01 | GSE149710 | GEO

RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with fructooligosaccharide

Project description:Purpose: Our data significantly advance understanding of Fructooligosaccharide regulatory mechanism in ileum of Taiping chicken Methods: Using RNA-seq analysis to study the gene expression in ileum tissue after Taiping chicken was given Fructooligosaccharide Results: A total of 164,629,826 and 152,047,552 raw reads were generated from the CT and FOS, respectively . After removing the interference data, about 164,028,872 and 151,452,588 clean reads were obtained Conclusions: through high-throughput sequencing of the six libraries from ileum of Taiping chicken, the expression level of mRNA has significant changes after given probiotics

2023-05-19 | GSE150908 | GEO

RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic

Project description:RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic

| PRJNA629783 | ENA

RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with fructooligosaccharide

Project description:RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with fructooligosaccharide

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Transcription profiling of rat colon after dietary treatment with fructo oligosaccharides

Project description:To increase our knowledge of the effects of Fructo oligosaccharides (FOS) on the intestinal barrier function in rats, a controlled rat infection study was performed. Two groups of rats were adapted to a diet with or without FOS. mRNA was collected from the mucosa of the colon and changes in gene expression were assessed using an agilent rat whole genome microarray (G4131A Agilent Technologies). Results indicate that dietary FOS influences energy metabolism, which will most likely play a role in the effects of FOS on the intestinal barrier. Experiment Overall Design: In the present study, large-scale gene expression analysis was performed to reveal mechanistic details of FOS induced gene expression in vivo in the colon mucosa. Wistar rats were adapted to diets with or without FOS for 14 days. RNA was isolated from colonic mucosal scrapings. Agilent rat whole genome microarray containing 44290 60-mer spots, were used to study FOS induced gene expression changes in order to better understand the FOS induced effects on the intestinal barrier of rats.

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Transcription profiling of rat cecum mucosa upon dietary treatment with fructo oligosaccharides

Project description:To increase our knowledge of the effects of Fructo oligosaccharides (FOS) on the intestinal barrier function in rats, a controlled rat infection study was performed. Two groups of rats (n=12 per group) were adapted to a diet with or without FOS. mRNA was collected from the mucosa of the cecum and changes in gene expression were assessed using an agilent rat whole genome microarray (G4131A Agilent Technologies). Results indicate that dietary FOS influences immune response and wound healing mechanisms, which will most likely affect the intestinal barrier. Experiment Overall Design: In the present study, large-scale gene expression analysis was performed to reveal mechanistic details of FOS induced gene expression in vivo in the cecum mucosa. Wistar rats were adapted to diets with (n=12) or without FOS (n=12) for 14 days. RNA was isolated from cecum mucosal scrapings, two RNA samples from the control group were ecluded based on poor quality of RNA. Agilent rat whole genome microarray containing 44290 60-mer spots, were used to study FOS induced gene expression changes in order to better understand the FOS induced effects on the intestinal barrier of rats.

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Colonic gene expression upon dietary treatment

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2007-12-31 | GSE8587 | GEO

Cranberry proanthocyanidins and dietary oligosaccharides synergistically modulate Lactobacillus plantarum physiology

Project description:Plant-based foods contain bioactive compounds such as polyphenols that resist digestion and potentially benefit the host through interactions with their gut microbiome. Based on previous observations, we hypothesized thatprobiotic Lactobacillus plantarum interact with cranberry polyphenols and dietary oligosaccharides to synergistically impact its physiology. In this study, L. plantarum ATCC BAA-793 was grown on dietary oligosaccharides including cranberry xyloglucans, fructooligosaccharides, and human milk oligosaccharidesin conjunction with proanthocyanidins (PACs) extracted from cranberry. As a result, L. plantarum exhibits a differential physiological response to cranberry PACs dependent on the carbohydrate source and polyphenol fraction introduced. Of two extracts evaluated, the PAC1 fraction increased growth regardless of oligosaccharide whereas PAC2 positively modulates growth during xyloglucan metabolism. Interestingly, PAC1 enables ATCC BAA-793 to utilize fructooligosaccharides efficiently as it is unable to ferment this substrate ordinarily. Relative to glucose, oligosaccharide metabolism increases the ratio of secreted acetic acid to lactic acid. The PAC2 fraction differentially increases this ratio during cranberry xyloglucan fermentation compared with PAC1. RNA-seq transcriptomics link expression of putative polyphenol degradation genes, polyphenol degradation profiles, and physiological phenotypes.

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