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Ralstonia solanacearum strain:Single bacteria in bacterial culture | isolate:Single bacteria in bacterial culture

ABSTRACT: Ralstonia solanacearum strain:Single bacteria in bacterial culture | isolate:Single bacteria in bacterial culture Genome sequencing

PROVIDER: PRJNA640736 | ENA |

REPOSITORIES: ENA

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Ralstonia solanacearum strain:Single bacteria in bacterial culture | isolate:Single bacteria in bacterial culture

Project description:Ralstonia solanacearum strain:Single bacteria in bacterial culture | isolate:Single bacteria in bacterial culture Genome sequencing

| PRJNA640744 | ENA

RNA-seq analysis of Ralstonia solanacearum treated with nitrosative and oxidative stress

Project description:Ralstonia solancearum causes bacterial wilt disease on diverse plant hosts. R. solanacearum cells enter a host from soil or infested water through the roots, then multiply and spread in the water-transporting xylem vessels. Despite the low nutrient content of xylem sap, R. solanacearum grows extremely well inside the host, using denitrification to respire in this hypoxic environment. R. solanacearum growth in planta also depends on the successful deployment of protein effectors into host cells using a Type III Secretion System (T3SS). The T3SS is absolutely required for R. solanacearum virulence, but it is metabolically costly and can trigger host defenses. Thus, the pathogen’s success depends on optimized regulation of the T3SS. We found that a byproduct of denitrification, the toxic free-radical nitric oxide (NO), positively regulates the R. solanacearum T3SS both in vitro and in planta. Using chemical treatments and R. solanacearum mutants with altered NO levels, we show that the expression of a key T3SS regulator is induced by NO in culture. Analyzing the transcriptome of R. solanacearum responding to varying levels of NO both in culture and in planta revealed that the T3SS and effectors were broadly upregulated with increasing levels of NO. This regulation was specific to the T3SS and was not shared by other stressors. Our results suggest that R. solanacearum experiences an NO-rich environment in the plant host and may use this NO as a signal to activate T3SS during infection.

2020-10-26 | GSE160024 | GEO

Expression data from Ralstonia solanacearum-inoculated tomato stem

Project description:Bacterial wilt caused by Ralstonia solanacearum is a lethal, soil-borne disease of tomato. Control of the disease with chemicals and crop rotation is insufficient, because the pathogen is particularly well adapted for surviving in the soil and rhizosphere. Therefore, cultivar resistance is the most effective means for controlling bacterial wilt, but the molecular mechanisms of resistance responses remain unclear. We used microarrays to obtain the characteristics of the gene expression changes that are induced by R. solanacearum infection in resistant cultivar LS-89 and susceptible cultivar Ponderosa.

2012-10-06 | GSE31807 | GEO

Ralstonia solanacearum strain GMI1000: Methylation profile

Project description:The aim of the project is to decipher the role of DNA methylation in the plant pathogenic bacteria Ralstonia solanacearum during host adaptation. As a first step, we present here the DNA methylation profile of the GMI1000 reference strain.

2017-01-10 | GSE93317 | GEO

Gene expression analysis of Mulberry root after inoculation with Ralstonia solanacearum.

Project description:Bacterial wilt, caused by the soil-borne bacterium Ralstonia solanacearum, is a lethal disease of mulberry, but the molecular mechanisms of the host resistance responses to R. solanacearum remain unclear. In order to better understand molecular resistance mechanisms to R. solanacearum in mulberry, we set out to define the changes in gene expression of resistance and susceptible mulberry cultivars after inoculation with R. solanacearum. Susceptible cultivar YSD10, resistance cultivar KQ10 and YS283 were inoculation with R. solanacearum, mulberry root samples were collected at 1 dpi and non-treated control in all cultivars. Then we performed RNA-Seq analyses on all mulberry root samples using Illumina HiSeq 2000.

2019-06-13 | GSE60030 | GEO

Expression analysis of Ralstonia solanacearum strain GMI1000 at 20°C and 28°C in rich medium (CPG) and in planta

Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain GMI1000 at 20°C and 28°C in culture and in planta. The tropical strain GMI1000 cannot wilt tomato plants at 20°C although it can cause full-blown disease at 28°C.

2012-01-15 | GSE33657 | GEO

Transcriptome analysis of 'Candidatus Liberibacter solanacearum' in its psyllid vector, Bactericera cockerelli

Project description:We seqeunced mRNA from the bacterial pathogen 'Candidatus Liberibacter solanacearum" during its association with the psyllid vector Bactericera cockerelli.

2014-07-21 | GSE57808 | GEO

Expression analysis of Ralstonia solanacearum strain UW551 at 20°C and 28°C in rich medium (CPG) and in planta

Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain UW551 at 20°C and 28°C in culture and in planta. The temperatel strain UW551 can wilt and cause full-blown disease on tomato plants at 28°C as well as at 20°C.

2012-01-15 | GSE33661 | GEO

Transcriptome analysis of 'Candidatus Liberibacter solanacearum' in its psyllid vector, Bactericera cockerelli [sexed insects]

Project description:We sequenced mRNA from the bacterial pathogen 'Candidatus Liberibacter solanacearum" during its association with the psyllid vector Bactericera cockerelli.

2017-02-09 | GSE81209 | GEO

interaction with bacteria or bacterial supernatant

Project description:Epithelial cells were in contact either with live bacteria (S. aureus) or with bacterial supernatant during 3 hours. Three dependant experiment for each condition were performed. RNA from epithelial cells were extracted. Each condition was hybridized on 6 slides (3 slides with their corresponding swap) Keywords: parallel sample

2004-10-23 | GSE1853 | GEO

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