Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Modulation of microRNA processing by 5-lipoxygenase

ABSTRACT: Modulation of microRNA processing by 5-lipoxygenase

PROVIDER: PRJNA640805 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12060501.fastq.gz	Fastqsanger.gz
	SRR12060502.fastq.gz	Fastqsanger.gz
	SRR12060503.fastq.gz	Fastqsanger.gz
	SRR12060504.fastq.gz	Fastqsanger.gz
	SRR12060505.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Modulation of microRNA processing by 5-lipoxygenase

Project description:Here, we describe the modulation of miRNA processing as a novel noncanonical function of the 5-lipoxygenase (5-LO) enzyme in monocytic cells. In differentiated Mono Mac 6 (MM6) cells, we found an in situ interaction of 5-LO with Dicer, a key enzyme in miRNA biogenesis. RNA sequencing of small non-coding RNAs revealed a functional impact, knockout of 5-LO altered the expression profile of several miRNAs. Our observations suggest that 5-LO regulates the miRNA profile by modulating the Dicer-mediated processing of distinct pre-miRNAs. 5-LO inhibits the formation of let-7e which induces cell differentiation and promotes the generation of the oncomirs miR-99b and miR-125a which induce cell proliferation and the maintenance of leukemic stem cell functions.

2020-10-30 | GSE152901 | GEO

A quantitative map of human primary microRNA processing sites

Project description:Maturation of canonical microRNA (miRNA) is initiated by DROSHA that cleaves the primary transcript (pri-miRNA). Over 1,800 miRNA loci are annotated in humans, but it remains largely unknown if and at which sites the pri-miRNAs are cleaved by DROSHA. Here we performed in vitro processing on a full set of human pri-miRNAs (miRBase v21) followed by sequencing. This comprehensive profiling enabled us to classify miRNAs based on DROSHA-dependence and map their cleavage sites with respective processing efficiency measures. Only 758 pri-miRNAs are confidently processed by DROSHA, while the majority may be non-canonical or false entries. Analyses of the DROSHA-dependent pri-miRNAs show key cis-elements for processing. We observe widespread alternative processing as well as unproductive cleavage events such as “nick” or “inverse” processing. SRSF3 is a broad-acting auxiliary factor modulating alternative processing and suppressing unproductive processing. The profiling data and methods developed in this study will allow systematic analyses of miRNA regulation.

2021-07-28 | PXD025767 | Pride

Systematic discovery of RNA binding proteins that control microRNA processing

Project description:We report the modulation of miRNA processing by several RNA binding proteins through the implementation of a high-throughput computational screen of eCLIP data

2018-04-11 | GSE102497 | GEO

Modulation of food microbiome by processing of apple fruits

Project description:Modulation of food microbiome by processing of apple fruits

| PRJEB48252 | ENA

An optimized microRNA scaffold increases shRNA processing and target knockdown

Project description:Two shRNAs were placed into expression vectors harboring mir30 microRNA scaffold and an optimized scaffold where the artificial restriction sights in mir30 have been removed. After infection and selection shRNA processing was assessed by small-RNA cloning. For both shRNAs, placement into the optimized scaffold resulted in a ~two-fold increase in processing (based on smallRNA levels).

2014-11-24 | GSE62187 | GEO

A quantitative map of human primary microRNA processing sites [1]

2021-07-27 | GSE174143 | GEO

A quantitative map of human primary microRNA processing sites [2]

2021-07-27 | GSE174222 | GEO

A herpesvirus microRNA targets host microRNA processing to promote virus reactivation from latency

Project description:A herpesvirus microRNA targets host microRNA processing to promote virus reactivation from latency

| PRJNA745355 | ENA

A quantitative map of human primary microRNA processing sites

Project description:A quantitative map of human primary microRNA processing sites

| PRJNA728872 | ENA

Project description:Age-related brainstem degeneration through microRNA modulation in mice