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AICAR regulate IEGs expression in ALL cells

ABSTRACT: AICAR regulate IEGs expression in ALL cells

PROVIDER: PRJNA640928 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12062627.fastq.gz	Fastqsanger.gz
	SRR12062628.fastq.gz	Fastqsanger.gz
	SRR12062629.fastq.gz	Fastqsanger.gz
	SRR12062630.fastq.gz	Fastqsanger.gz

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AICAR regulate IEGs expression in ALL cells

Project description:To identify genes transcriptionally regulated by AICAR under cellular stress, We treated NALM6 cells for 45 min with vehicle (DMSO) or AICAR (15 mM), and examined their RNA profile using RNA-Seq. The immediate early genes (IEGs) were identified as a subset of genes downregulated by AICAR.

2021-07-22 | GSE152917 | GEO

TNF-a expression by myeloid cells in ascites regulate colorectal cancer

Project description:Interventions: Colorectal cancer surgery inguinal hernia surgery Primary outcome(s): TNF-a expression by myeloid cells in ascites Study Design: Parallel Non-randomized

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AICAR can modulate the expression of microRNA in J1 mESC

Project description:We have demonstrated AICAR can maintain J1 mouse ES cells pluripotency in our previous research, yet its effects on ES cells miRNAs expression remain a mystery. In this study, we performed small RNA (sRNA) High-throughput sequencing using Illumina HiSeq 2000 to investigate the influence of AICAR on J1 mouse ES cells miRNAs expression and further found the mechanism of how miRNAs affect ES cells pluripotency maintenance. Samples that treated by DMSO is used as a control.

2014-02-06 | GSE54586 | GEO

Insulin and 5-aminoimidazole-4-carboxamide Ribonucleotide (AICAR) Differentially Regulate the Skeletal Muscle Cell Secretome

Project description:Skeletal muscle is a major contributor to whole-body glucose homeostasis and is an important endocrine organ. To date, few studies have undertaken the large-scale identification of skeletal muscle-derived secreted proteins (myokines), particularly in response to stimuli that activate pathways governing energy metabolism in health and disease. Whereas the AMP-activated protein kinase (AMPK) and insulin-signaling pathways have received notable attention for their ability to independently regulate skeletal muscle substrate metabolism, little work has examined their ability to re-pattern the secretome. The present study coupled the use of high-resolution MS-based proteomics and bioinformatics analysis of conditioned media derived from 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR – an AMPK activator)- and insulin-treated differentiated C2C12 myotubes. We quantified 858 secreted proteins, including cytokines and growth factors such as fibroblast growth factor-21 (Fgf21). We identified 377 and 118 proteins that were significantly altered by Insulin and AICAR treatment, respectively. Notably, the family of insulin growth factor binding-proteins (Igfbp) was differentially regulated by each treatment. Insulin- but not AICAR-induced conditioned media increased the mitochondrial respiratory capacity of myotubes, potentially via secreted factors. These findings may serve as an important resource to elucidate secondary metabolic effects of insulin and AICAR stimulation in skeletal muscle.

2021-09-15 | PXD025687 | Pride

Effect of AICAR monophosphate and AICAr riboside accumulation on global transcription

Project description:AICAR monophosphate (5-Aminoimidazole-4-carboxamide ribonucleotide) is a metabolic intermediate of the de novo purine synthesis pathway presenting highly promising metabolic and antiproliferative properties. Yeast mutants accumulating AICAR are auxotroph for histidine. A screening for suppressors of this phenotype identified recessive and dominant mutants that result in lowering intracellular AICAR concentration. The recessive mutants affect adenosine kinase which is shown here to catalyze the phosphorylation of AICAR riboside in yeast. The dominant mutants strongly enhance the capacity of the alkaline phosphatase Pho13p to dephosphorylate succinyl-AICAR monophosphate into the non-toxic riboside form. By combining these mutants to transcriptomic and metabolomic analyses, we establish that in yeast both toxic and physiological responses to AICAR are linked to the concentration of the monophosphate form, while the nucleoside moiety has no effect even at high concentration. Finally, we establish that (S)AICAR concentration varies under physiological conditions, thus modulating transcriptional regulation of purine pathway genes. This SuperSeries is composed of the SubSeries listed below.

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RNA-seq of U937 cells treated with AICAr

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AICAR can modulate the expression of microRNA in J1 mESC

2014-02-06 | E-GEOD-54586 | biostudies-arrayexpress

Expression data from Daudi cells stimulated with anti-CD40 and/or IL-4 for 6 hr with or without AICAR-pretreatment

Project description:We used microarrays to detail the action of AICAR on the transcriptome response to CD40-ligation and IL-4 stimulation in Daudi cells. Keywords: gene expression array-based, response to anti-CD40 and/or IL-4 with or without AICAR

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Transcriptome of primary acute myeloid leukemia (AML) cells treated with AICAr

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