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Expression data from isorhamnetin (Iso)-treated human amnion epithelial stem cells (hAESCs)

ABSTRACT: Expression data from isorhamnetin (Iso)-treated human amnion epithelial stem cells (hAESCs)

PROVIDER: PRJNA641631 | ENA |

REPOSITORIES: ENA

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Expression data from isorhamnetin (Iso)-treated human amnion epithelial stem cells (hAESCs)

Project description:Gene expression profiling reveals a potential role of Iso towards hepatic differentiation of hAESCs. hAESCs were isolated from discarded term placenta and were treated with 20 μM Iso for 10 days. Microarray gene expression profiling was conducted for biological replicates of Iso-treated hAESCs on day 10 and untreated control cells on day 0, and day 10.

2020-06-25 | GSE153149 | GEO

Expression data from isorhamnetin (Iso)-treated human amnion epithelial cells (hAECs)

Project description:Expression data from isorhamnetin (Iso)-treated human amnion epithelial cells (hAECs)

| PRJNA625696 | ENA

Expression data from isorhamnetin (Iso)-treated human amnion epithelial cells (hAECs)

Project description:Gene expression profiling reveals a potential role of Iso towards hepatic differentiation of hAECs.

2020-04-17 | GSE148777 | GEO

Expression data from NASH-induced mouse liver treated with isorhamnetin

Project description:Gene expression profiling reveals a potential role of isorhamnetin in the mitigation of NASH features including steatosis, liver injury, and fibrosis Microarray gene expression profiling was conducted for technical replicates of healthy liver as control (CTL), NASH-induced (NASH), NASH-induced treated with isorhamnetin for 14 days (50 mg/kg of body weight) (NASH+ISO) liver tissues to identify its effect in the regulation of pathways involved in pathologic features of NASH.

2019-09-19 | GSE137635 | GEO

Expression data from Verbenalin-treated human amnion epithelial cells (hAECs)

Project description:Expression data from Verbenalin-treated human amnion epithelial cells (hAECs)

| PRJNA564436 | ENA

Human amnion epithelial cell therapy reduces hypertension-induced vascular stiffening and cognitive impairment

Project description:We investigated the effect of amnion epithelial cells on hypertension-induced transcriptomic changes in the brain

2023-12-30 | GSE248059 | GEO

Isorhamnetin Inhibits Proliferation and Invasion and Induces Apoptosis Through the Mitochondria‑Dependent Apoptosis in Human Gastric Cancer Cells

Project description:The major treatment strategies for gastric cancer (GC) including surgery, chemotherapy, and molecular-targeted therapy are limited by tumor recurrence and chemoresistance. Isorhamnetin (ISO) derives from the total flavones of Hippophae rhamnoides L. and has been used as potential antitumor medicine in several types of cancer. However, the antitumor effect of ISO on GC cells remains unclear. Here, we investigated the effects of ISO on two types of GC cells and explored its apoptotic mechanisms underlying such effects. The transcriptomic analysis of ISO-treated GC cells enriched multiple targets on cell growth and apoptosis. The CCK-8, colony formation and morphology observation assays demonstrated that ISO treatment significantly inhibited the growth and proliferation of GC cells in time- and dose-dependent manners. In addition, we also found that ISO treatment inhibited cell migration and invasion. Furthermore, ISO increased TUNEL positive apoptotic bodies as well as severe cell apoptosis. Intriguingly, ISO treatment significantly increased the expression of mitochondria-associated Bax/Bcl-2, cytosolic Cytochrome c and activated the cleavage of Caspase-3 and PARP, followed by severe reduction of mitochondrial potential. Moreover, the apoptosis of ISO-treated GC cells was blocked by the specific inhibitor of Caspase-3. Therefore, we demonstrated that ISO could induce apoptosis of GC cells through mitochondria-dependent apoptotic pathway. In summary, we reported for the first time the antitumor function of ISO in GC via intervening apoptosis, indicating a potential application in GC therapy.

2022-05-30 | GSE204967 | GEO

Expression data from rosmarinic acid-treated human amnion epithelial cells (hAECs)

Project description:Expression data from rosmarinic acid-treated human amnion epithelial cells (hAECs)

| PRJNA550970 | ENA

Transcription profiling of human amnion epithelial FL cells treated with benzo(a)pyrene diol epoxide

Project description:Genotoxic agents cause cellular DNA damage and stress responses, including transcriptional changes. Here we focused on the early transcriptional responses of human cells to benzo(a)pyrene diol epoxide (BPDE), which causes bulky DNA adduct damage. Human amnion epithelial FL cells were exposed to three doses of BPDE (5, 50, and 500 nM) and the vehicle control DMSO, and differential gene expression profiles were obtained 4 h after exposure using oligonucleotide microarrays followed by validation with quantitative real-time RT-PCR. Compared with a few genes affected by the low and medium-dose exposure, extensive and robust changes in gene expression were induced by the high-dose BPDE. We found that the expression of cell cycle-regulators, signaling molecules and transcription factors were significantly altered and important signaling pathways related to cell survival or apoptosis were affected by BPDE. Several genes and related regulatory pathways that were previously not known to be responsive to this genotoxic agent have now been implicated, which helps to draw the whole picture of how cells respond to environmental chemical exposure via transcriptional regulation. Experiment Overall Design: Human amnion epithelial FL cells were exposed to vehicle control (dimethyl sulfoxide) and increasing doses (5, 50, 500 nM) of anti-benzo(a)pyrene diol epoxide (anti-BPDE), respectively. The transcriptomes of the three treatments were compared to that of the control, respectively, to test the hypothesis that a characterized differential expression profile would be generated by exposure to various doses of this genotoxic agent.

2008-04-08 | E-GEOD-7675 | biostudies-arrayexpress

Transcription profiling by array of human amnion epithelial FL cells treated with the carcinogen BPDE at low concentration

Project description:The environmental carcinogen, (±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), causes bulky-adduct DNA damages, triggers certain signaling pathways, and elicits gene expression changes. Here, we focused on the temporal gene expression changes induced by a low concentration (0.05 µM) BPDE in human amnion epithelial FL cells. Differential gene expression profiles at 1, 10 and 22 h post BPDE treatment were obtained using Affymetrix HG-U133 Plus 2.0 oligonucleotide microarrays. A cohort of gene expression changes related to cell cycle progression, cell growth or apoptosis, stress response, and post-transcriptional regulation was validated with quantitative real-time RT-PCR. The alteration of several cell cycle-related genes was correlated and possibly contributed to the cell cycle arrest phenotype. Paradoxical transcriptional regulations regarding cell growth or apoptosis emerged in response to BPDE treatment, which indicated that cell fate was determined by integrated signals. The temporal transcriptional changes would be of help to clarify the molecular mechanism of cellular response to BPDE. Experiment Overall Design: Human amnion epithelial FL cells were exposed to vehicle control (dimethyl sulfoxide) and a low concentration (0.05 µM) (±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide, respectively. The differential gene expression profiles at 1, 10 and 22 h post BPDE treatment were obtained using Affymetrix HG-U133 Plus 2.0 oligonucleotide microarrays. The transcriptomic changes at different time points post BPDE treatment would provide insight into the dynamic processes of cellular response to this genotoxic agent.

2009-01-14 | E-GEOD-10979 | biostudies-arrayexpress

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