Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: bacteria of oral-nasal-pharyngeal samples of mouth breathing children Raw sequence reads

PROVIDER: PRJNA642373 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12370000_1.fastq.gz	Fastqsanger.gz
	SRR12370000_2.fastq.gz	Fastqsanger.gz
	SRR12370100_1.fastq.gz	Fastqsanger.gz
	SRR12370100_2.fastq.gz	Fastqsanger.gz
	SRR12370001_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 320

Similar Datasets

Saliva LC-MS/MS of mouth breathing children

Project description:Salivary proteins of mouth breathing children were compared with paired nose breathing children using label-free quantification (LFQ). The differentially expressed proteins were screened (fold-change 1.5, p value<0.05).

2021-09-09 | PXD021106 | Pride

Host Gene Expression in Nasal and Blood Samples for the Diagnosis of Viral Respiratory Infection

Project description:Assessment of host gene expression is an emerging tool for the diagnosis of human infections. We compared nasal and blood samples for evaluation of the host transcriptomic response in children with acute respiratory syncytial virus (RSV), symptomatic and asymptomatic picornavirus (PV) infection, and virus-negative asymptomatic controls (Ctrls). RNA was extracted from nasal and blood samples and analyzed by microarray. Despite generally lower quality of nasal RNA, the number of genes detected in each sample type was equivalent. Nasal gene expression signal derived mainly from epithelial cells but also included a leukocyte contribution that was higher in samples from symptomatic children. The number of genes with increased expression in virus-infected children was comparable in nasal and blood samples, while nasal samples also had large numbers of genes with decreased expression, including many genes associated with ciliary function and assembly. Compared to symptomatic children, those with asymptomatic PV had fewer genes with increased or decreased expression in both sample types. Genes with increased expression in comparisons of symptomatic children versus Ctrls included genes associated with components of innate immunity and apoptosis. Children with RSV but not PV also had increased expression of genes related to the cell cycle. Using nested leave-one-pair-out cross-validation and supervised principal components analysis, we defined sets of genes whose expression patterns accurately classified subjects, with high area-under-the-curve values in receiver operating characteristic analysis. Our results support use of nasal samples to augment pathogen-based tests to diagnose viral respiratory infection.

2019-01-01 | GSE117827 | GEO

human nasopharyngeal metagenome

Project description:human nasal/pharyngeal metagenome

| PRJNA338893 | ENA

Project description:Nasal pharyngeal swab metagenomics

| PRJNA613697 | ENA

Project description:Nasal pharyngeal swab viral metagenomics

| PRJNA701833 | ENA

oral bacteria

Project description:oral bacteria Raw sequence reads

| PRJNA848441 | ENA

Gene expression profiling of nasal swabs samples from children with asthma or wheezing exacerbations.

Project description:Nasal swab specimens were collected from children who presented to the emergency department with an acute exacerbation of asthma or wheeze. Samples were also collected from control subjects. Convalescent/quiescent samples were collected from children who were followed-up at least 6 weeks after an acute exacerbation of asthma or wheeze. Gene expression was profiled on microarrays.

2019-02-17 | GSE103166 | GEO

Evidence for proline utilisation by oral bacterial biofilms grown in saliva

Project description:Within the mouth bacteria are starved of saccharides as their main nutrient source between meals and it is unclear what drives their metabolism. Previously oral in vitro biofilms grown in saliva have shown proteolytic degradation of salivary proteins and increased extracellular proline. Although arginine and glucose have been shown before to have an effect on oral biofilm growth and activity, there is limited evidence for proline. Nuclear magnetic resonance (NMR) spectroscopy was used to identify extracellular metabolites produced by bacteria in oral biofilms grown on hydroxyapatite discs. Biofilms were inoculated with whole mouth saliva and then grown for 7 days using sterilised whole mouth saliva supplemented with proline, arginine and glucose as a growth-medium. Overall proline had a beneficial effect on biofilm growth – with significantly fewer dead bacteria present by biomass and surface area of the biofilms (p <0.05). Where arginine and glucose significantly increased and decreased pH, respectively, the pH of proline supplemented biofilms remained neutral at pH 7.3-7.5. SDS-polyacrylamide gel electrophoresis of the spent saliva from proline and arginine supplemented biofilms showed inhibition of salivary protein degradation of immature biofilms. NMR analysis of the spent saliva revealed that proline supplemented biofilms were metabolically similar to unsupplemented biofilms, but these biofilms actively metabolised proline to 5-aminopentanoate, butyrate and propionate, and actively utilised glycine. This study shows that in a nutrient limited environment, proline has a beneficial effect on in vitro oral biofilms grown from a saliva inoculum.

2020-07-23 | ST001433 | MetabolomicsWorkbench

Project description:Perioperative nasal microbiome samples - raw sequence reads

| PRJNA342328 | ENA

Project description:oral resident bacteria Raw sequence reads

| PRJNA356789 | ENA