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M6A modification maps of mRNA in bone marrow-derived macrophages (BMDMs) from mouse

ABSTRACT: m6A modification maps of mRNA in bone marrow-derived macrophages (BMDMs) from mouse

PROVIDER: PRJNA642873 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12110267.fastq.gz	Fastqsanger.gz
	SRR12110268.fastq.gz	Fastqsanger.gz
	SRR12110269.fastq.gz	Fastqsanger.gz
	SRR12110270.fastq.gz	Fastqsanger.gz
	SRR12110271.fastq.gz	Fastqsanger.gz

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m6A modification maps of mRNA in bone marrow-derived macrophages (BMDMs) from mouse

Project description:We report the application of sequencing technology for high-throughput profiling of mRNA m6A methylation in mice BMDMs with or without PBS treatment. By obtaining over thirty billion bases of sequence from m6A antibody immunoprecipitated mRNA, we generated m6A methylation maps of mouse BMDMs in the presence or absence of LPS. We find that SOCS1 mRNA methylation is robustly enhanced by LPS treatment in WT BMDMs, leading to the increase of SOCS1 expression. However, LPS challenge fails to increase SOCS1 expression in the M14-/- BMDMs, indicating m6A modification plays a cirital role in the SOCS1 expression. This study provides a framework for the application of comprehensive mRNA m6A profiling towards characterization of BMDMs.

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Project description:To investigate the role of METTL3-mediated m6A modification in macrophage, we performed m6A-sequencing to map the m6A modification in bone-marrow-derived macrophages (BMDMs) in wild type (WT) and Mettl3-/- mice.

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Project description:To investigate the role of RNA methyltransferase METTL3-mediated m6A modification in macrophage, we performed m6A-sequencing to map the m6A modification in bone-marrow-derived macrophages (BMDMs) from Mettl3fl/fl (WT) and Mettl3fl/fl,LyzM-cre (cKO) mice

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