Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Assessment of Pre-Analytical Sample Handling Conditions for Comprehensive Liquid Biopsy Analysis

PROVIDER: PRJNA643393 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12133221.fastq.gz	Fastqsanger.gz
	SRR12133222.fastq.gz	Fastqsanger.gz
	SRR12133223.fastq.gz	Fastqsanger.gz
	SRR12133224.fastq.gz	Fastqsanger.gz
	SRR12133225.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 15

Similar Datasets

Analysis of gliomas DNA methylation: Assessment of pre-analytical variables

Project description:Analysis of gliomas DNA methylation: Assessment of pre-analytical variables

| PRJNA1167815 | ENA

Homo sapiens

Project description:Impact of pre-analytical handling on neuroblastoma cells mRNA gene expression

| PRJNA267205 | ENA

Pre-analytical changes of mRNA Gene Expression as the result from prolonged storage

Project description:Impact of pre-analytical handling on Bone Marrow (BM) mRNA Gene Expression: We have investigated the impact of RNA extraction protocols and time delays (24 and 48h) between sample aspiration and RNA extraction on RNA quality and gene expression profiles. Intact RNA can be extracted from BM samples stored at room temperature for up to 48h after aspiration. However, storage of BM has dramatic effects on mRNA expression of individual transcripts. Keywords: time-course

2004-04-28 | GSE1347 | GEO

Transcription profiling time series of human bone marrow samples stored at room temperature for up to 48 hours after aspiration

2007-07-28 | E-GEOD-1347 | biostudies-arrayexpress

Analysis of gliomas DNA methylation: Assessment of pre-analytical variables

Project description:MeDIP-Seq for assessment of the effect of pre-analytical variables on DNA methylation

2024-10-07 | GSE278575 | GEO

Pre-analytical factors affecting the establishment of a single tube assay for multi-parameter liquid biopsy detection in melanoma patients

Project description:Pre-analytical factors affecting the establishment of a single tube assay for multi-parameter liquid biopsy detection in melanoma patients

| PRJNA599356 | ENA

Online Nanoflow Two-Dimension Comprehensive Active Modulation Reversed Phase-Reversed Phase Liquid Chromatography High Resolution Mass Spectrometry for Metaproteomics of Environmen-tal and Microbiome Samples

Project description:Metaproteomics is a powerful analytical approach that can assess the functional capabilities deployed by microbial communi-ties in both environmental and biomedical microbiome settings. Yet the mass spectra resulting from these mixed biological communities are challenging due to the high number of low intensity peak features. The use of multiple dimensions of chroma-tographic separation prior to mass spectrometry analyses has been applied to proteomics previously but can require increased sampling handling and instrument time. Here we demonstrate an automated online comprehensive active-modulation two-dimensional liquid chromatography method for metaproteome sample analysis. A high pH PLRP-S column was used in the first dimension followed by low pH separation in the second dimension using dual modulating C18 traps and a C18 column. This method increased the number of unique peptides found in ocean metaproteome samples by more than 50% when com-pared to a one dimension separation while using the same amount of sample and instrument time.

2022-05-20 | PXD027351 | Pride

Impact of pre-analytical handling on neuroblastoma cells mRNA gene expression

Project description:Gene expression analyses of disseminated tumor cells can provide indispensable biological and clinical information at diagnosis, during therapy and at relapse. So far, however, disseminated tumor cells expression analyses have been hampered by insufficient infiltration rates and/or unclear consequences of transport duration, temperature and other pre-handling procedures. This study, using neuroblastoma as a model, focuses on the influences of pre-analytical procedures like tumor cell enrichment, transport time and temperature on the disseminated tumor cells expression profile.

2015-12-01 | GSE63294 | GEO

Project description:Comparing different sample handling conditions for 16S rRNA amplicon sequencing

| PRJNA420005 | ENA

Quality control in quantitative proteomics: a case study for the human CSF proteome

Project description:Proteome studies using mass spectrometry (MS)-based quantification is a main approach for the discovery of new biomarkers. However, a number of analytical conditions in front and during MS data acquisition can affect the accuracy of the obtained outcome. Therefore, comprehensive quality assessment of the acquired data plays a central role in quantitative proteomics, though due to immense complexity of MS data it is often neglected. Here, we practically address the quality assessment of quantitative MS data describing key steps for the evaluation including the levels: raw data, identification and quantification. With this, four independent datasets from cerebrospinal fluid, an important biofluid for neurodegenerative diseases biomarker studies, were assessed demonstrating that already sample processing-based differences are reflected on all three levels but with a varying impact on the quality of the quantitative data.

2023-04-12 | PXD037650 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data