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Next Generation Sequencing of Wild Type and Zeb1-/- CLP

ABSTRACT: Next Generation Sequencing of Wild Type and Zeb1-/- CLP

PROVIDER: PRJNA643569 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12129983_1.fastq.gz	Fastqsanger.gz
	SRR12129983_2.fastq.gz	Fastqsanger.gz
	SRR12129984_1.fastq.gz	Fastqsanger.gz
	SRR12129984_2.fastq.gz	Fastqsanger.gz
	SRR12129985_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing of Wild Type and Zeb1-/- CLP and HSCs

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Next Generation Sequencing of Wild Type and Zeb1-/- CLP

Project description:Zeb1, a zinc finger E-box binding homeobox epithelial-mesenchymal (EMT) transcription factor, confers properties of ‘stemness’, such as self-renewal, in cancer. Yet little is known about the function of Zeb1 in adult stem cells. Here, we used the hematopoietic system, as a well-established paradigm of stem cell biology, to evaluate Zeb1 mediated regulation of adult stem cells. We employed a conditional genetic approach using the Mx1-Cre system to specifically knockout (KO) Zeb1 in adult hematopoietic stem cells (HSCs) and their downstream progeny. Acute genetic deletion of Zeb1 led to rapid onset thymic atrophy and apoptosis driven loss of thymocytes and T cells. A profound cell-autonomous self-renewal defect and multi-lineage differentiation block was observed in Zeb1 KO HSCs. Loss of Zeb1 in HSCs activated transcriptional programs of deregulated HSC maintenance and multi-lineage differentiation genes, and of cell polarity, consisting of cytoskeleton, lipid metabolism/lipid membrane and cell adhesion related genes. Notably, Epithelial cell adhesion molecule (EpCAM) expression was prodigiously upregulated in Zeb1 KO HSCs, which correlated with their enhanced cell survival capacity and diminished differentiation in transplantation. Thus, Zeb1 acts as a crucial transcriptional regulator in hematopoiesis, co-ordinating HSC self-renewal and multi-lineage differentiation fates, in part, via EpCAM repression.

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Next Generation Sequencing of Wild Type and Zeb1-/- HSCs

Project description:Next Generation Sequencing of Wild Type and Zeb1-/- HSCs

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Next Generation Sequencing of Wild Type and Zeb1-/- HSCs

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Next Generation Sequencing of Wild Type and Zeb1-/- CLP and HSCs

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-07-02 | GSE153664 | GEO

Next Generation Sequencing of LSK EpCAM+ and EpCAM- of Zeb1-/-

Project description:Next Generation Sequencing of LSK EpCAM+ and EpCAM- of Zeb1-/-

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Next Generation Sequencing of LSK EpCAM+ and EpCAM- of Zeb1-/-

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Next Generation Sequencing Facilitates Quantitative Analysis of ZEB1-Regulated Transcriptomes

Project description:The goal of this study is to analyze the transcripts regulated by ZEB1.

2017-08-10 | GSE102337 | GEO

MS-based quantitative proteomic analysis of ribosomal fractions from wild-type and Zeb1-overexpressing human mammary epithelial cell

Project description:Epithelial-mesenchymal transition (EMT) involves profound changes in cell morphology, driven by transcriptional and epigenetic reprogramming. However, it emerges that translation and the ribosome composition play also key role in establishing physio-pathological phenotypes. Using genome-wide analyses, we report significant rearrangement of the translational landscape and machinery during EMT. Specifically, a mesenchymal cell line overexpressing the EMT transcription factor ZEB1 shows alterations in translational reprogramming and fidelity. Considering the change in translational activity of ZEB1-overexpressing mesenchymal cells, including in fidelity activity, we sought for changes in ribosome composition. We thus performed a riboproteome approach, i.e., mass spectrometry (MS)-based quantitative proteomic analysis of purified cytoplasmic ribosomes to highlight any change in relative amount of individual ribosomal proteins between wild-type and ZEB1-overexpressing human mammary epithelial cells.

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