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ABSTRACT: Microbial community structure of the anaerobic digestion system of rice straw and its main components

PROVIDER: PRJNA643639 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12131890_1.fastq.gz	Fastqsanger.gz
	SRR12131890_2.fastq.gz	Fastqsanger.gz
	SRR12131891_1.fastq.gz	Fastqsanger.gz
	SRR12131891_2.fastq.gz	Fastqsanger.gz
	SRR12131892_1.fastq.gz	Fastqsanger.gz

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Project description:Functional genes of the anaerobic digestion system of rice straw and its main components

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The microbial functional gene diversity in anaerobic digesters under different organic loadings

Project description:Anaerobic digestion is a popular and effective microbial process for waste treatment. The performance of anaerobic digestion processes is contingent on the balance of the microbial food web in utilizing various substrates. Recently, co-digestion, i.e., supplementing the primary substrate with an organic-rich co-substrate has been exploited to improve waste treatment efficiency. Yet the potential effects of elevated organic loading on microbial functional gene community remains elusive. In this study, functional gene array (GeoChip 5.0) was used to assess the response of microbial community to the addition of poultry waste in anaerobic digesters treating dairy manure. Consistent with 16S rRNA gene sequences data, GeoChip data showed that microbial community compositions were significantly shifted in favor of copiotrophic populations by co-digestion, as taxa with higher rRNA gene copy number such as Bacilli were enriched. The acetoclastic methanogen Methanosarcina was also enriched, while Methanosaeta was unaltered but more abundant than Methanosarcina throughout the study period. The microbial functional diversity involved in anaerobic digestion were also increased under co-digestion.

2017-01-12 | GSE93419 | GEO

Project description:Anaerobic digestion microorganism of rice straw

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Multi-level microbial fingerprinting in anaerobic digestion reveals consistent fingerprinting profiles

Project description:The anaerobic digestion microbiomes has been puzzling us since the dawn of molecular methods for mixed microbial community analysis. Monitoring of the anaerobic digestion microbiome can either take place via a holistic evaluation of the microbial community through fingerprinting or by targeted monitoring of selected taxa. Here, we compared four different microbial community fingerprinting methods, i.e., amplicon sequencing, metaproteomics, metabolomics and phenotypics, in their ability to reflect the full-scale anaerobic digestion microbiome. The phenotypic fingerprinting reflects a, for anaerobic digestion, novel, single cell-based approach of direct microbial community fingerprinting. Three different digester types, i.e., sludge digesters, digesters treating agro-industrial waste and dry anaerobic digesters reflected different operational parameters. The α-diversity analysis yielded inconsistent results, especially for richness, across the different methods. In contrast, β-diversity analysis resulted in comparable profiles, even when translated into phyla or functions, with clear separation of the three digester types. In-depth analysis of each method's features i.e., operational taxonomic units, metaproteins, metabolites, and phenotypic traits, yielded certain similar features yet, also some clear differences between the different methods, which was related to the complexity of the anaerobic digestion process. In conclusion, phenotypic fingerprinting is a reliable, fast method for holistic monitoring of the anaerobic digestion microbiome, and the complementary identification of key features through other methods could give rise to a direct interpretation of anaerobic digestion process performance.

2021-05-26 | PXD024788 | Pride

Project description:microbial community diversity in anaerobic co-digestion of swine manure and rice straw

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Project description:The microbial community in anaerobic digestion system

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GeoChip 5.0 microarray of the active bacterial community from both warming and control soil samples

Project description:To determine whether and how warming affects the functional capacities of the active microbial communities, GeoChip 5.0 microarray was used. Briefly, four fractions of each 13C-straw sample were selected and regarded as representative for the active bacterial community if 16S rRNA genes of the corresponding 12C-straw samples at the same density fraction were close to zero.

2023-12-30 | E-MTAB-13326 | biostudies-arrayexpress

Project description:Microbial community of anaerobic digestion of rice straw enhanced by amino acid ionic liquid

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Project description:Advanced anaerobic digestion study on microbial community structure

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Project description:The microbial community and enzymes in fermented rice using defined microbial starter, containing Rhizopus oryzae, Saccharomycopsis fibuligera, Saccharomyces cerevisiae and Pediococcus pentosaceus, play an important role in quality of the fermented rice product and its biological activities including melanogenesis inhibitory activity. The microbial metaproteome revealed large-scale proteins expressed by the microbial community to better understand the role of microbiota in the fermented rice.

2020-05-25 | PXD018081 | JPOST Repository

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