Project description:Organoids grown from intestinal crypts of controls and patients with Crohn's disease were sub-cultured at least 6 passages. RNA sequencing was performed for the 12 paired TNF-free and -treated control and Crohn's organoids.
Project description:Organoids grown from intestinal crypts of controls and patients with Crohn's disease were sub-cultured at least 6 passages. Single cell RNA sequencing was performed for the paired TNF-free and -treated control and Crohn's organoids.
Project description:RNA sequencing was performed for the TNF-free and -treated enteroids derived from control (n=8 pairs) and Crohn's disease patients (n=8 pairs).
Project description:RNA sequencing was performed for the TNF- or vehicle-treated organoids derived from the large intestine of healthy male C57BL/6J mice (n = 3 per group).
Project description:To address the effect of inflammatory stimulation on intestinal epithelial cells, we performed RNA-seq analysis on human colonic epithelial organoids treated with TNF and/or PGE2.
Project description:To investigate the direct effect of bleomycin on alveolar epithelium, feeder-free mouse alveolar organoids were treated by bleomycin (100μM) for 48 hours in vitro and then analyzed.