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ABSTRACT: Transcriptome analysis of Sphingomonas melonis TY

PROVIDER: PRJNA643919 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12142721_1.fastq.gz	Fastqsanger.gz
	SRR12142721_2.fastq.gz	Fastqsanger.gz
	SRR12142722_1.fastq.gz	Fastqsanger.gz
	SRR12142722_2.fastq.gz	Fastqsanger.gz
	SRR12142723_1.fastq.gz	Fastqsanger.gz

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sRNA participates in hyperosmotic stress response regulation in Sphingomonas melonis TY

Project description:Drought and salinity are most ubiquitous environmental factors that causing hyperosmotic threats to Sphingomonas and impairs their efficiency of performing environmental functions. However, bacteria have developed various responses and regulation systems to coping with these abiotic challenges. Among which post-transcriptional regulation plays vital roles in regulating gene expression and cellular homeostasis, as hyperosmotic stress conditions could lead to induction of specific small RNA (sRNA) that participates in stress response regulation. Here, we report a candidate functional sRNAs landscape of S. melonis which could help for comprehensive analyses of sRNA regulation in Sphingomonas species. WGCNA analysis revealed a 263 nt sRNA SNC251 which transcribed from its own promoter and shew the most dramatic correlation coefficient with hyperosmotic factors was characterized. An in vivo translation-reporter system constructed in this study revealed positive regulation and feedback mechanisms between the SNC251 and nicotine degradation genes. Deletion of snc251 affected multiple cellular processes and nicotine degradation capacity of S. melonis TY, while overexpression of SNC251 facilitated the biofilm formation capability of TY under hyperosmotic stress. Two genes of TonB system were further verified could be activated by SNC251, which also means SNC251 is a trans-acting small RNA. Briefly, this research reported a summary of sRNAs which participate in hyperosmotic stress response in S. melonis TY and revealed a novel sRNA SNC251 which is necessary for hyperosmotic stress response.

2023-02-07 | GSE224545 | GEO

Global transcriptional regulation of Sphingomonas melonis TY in response to hyperosmotic stress

Project description:To further elucidate the responsive patterns of S. melonis TY to hyperosmotic stress at transcriptional level, transcriptome sequencings were carried out on S. melonis TY cultured under three levels of salt stress (0%, 1%, 2%) for 10 or 30 minutes.

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sRNA137 regulation in Sphingomonas melonis TY

Project description:sRNA137 regulation in Sphingomonas melonis TY

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Project description:Sphingomonas melonis TY to heat stress

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sRNA137 regulation in Sphingomonas melonis TY

Project description:Transcriptome of the wild type (WT) of S. melonis TY and snc137 deleted cells carrying either a plasmid overexpressing SNC137 (Δsnc137 comp.), or an empty control vector (Δsnc137)

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Sphingomonas melonis TY strain:TY

Project description:Sphingomonas melonis TY strain:TY Genome sequencing and assembly

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Sphingomonas melonis TY strain:TY

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Project description:Transcriptional regulation of Sphingomonas melonis TY in response to hyperosmotic stress

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sRNA participates in hyperosmotic stress response regulation in Sphingomonas melonis TY

Project description:sRNA participates in hyperosmotic stress response regulation in Sphingomonas melonis TY

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Leaf transcriptomes of Arabidopsis thaliana colonized by representative phyllosphere commensals alone and in combination with the pathogen Pseudomonas syringae DC3000

Project description:Plants are colonized by a variety of microorganisms, the plant microbiota. In the phyllosphere, the above-ground parts of plants, bacteria are the most abundant inhabitants. Most of these microorganisms are not pathogenic and the plant responses to commensals or to pathogen infection in the presence of commensals are not well understood. We report the Arabidopsis leaf transcriptome after 3 to 4 weeks of colonization by Methylobacterium extorquens PA1 and Sphingomonas melonis Fr1, representatives of two abundant genera in the phyllosphere, compared to axenic plants. In addition, we also sequenced the transcriptome of Arabidopsis 2 and 7 days after spray-infection with a low dose of P. syringae DC3000 and in combination with the commensals.

2016-06-05 | E-MTAB-4683 | biostudies-arrayexpress

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