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ChIP-seq analysis reveals the direct targets of GltR in the P. aeruginosa genome

ABSTRACT: ChIP-seq analysis reveals the direct targets of GltR in the P. aeruginosa genome

PROVIDER: PRJNA644345 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12152387_1.fastq.gz	Fastqsanger.gz
	SRR12152387_2.fastq.gz	Fastqsanger.gz
	SRR12152388_1.fastq.gz	Fastqsanger.gz
	SRR12152388_2.fastq.gz	Fastqsanger.gz
	SRR12152389_1.fastq.gz	Fastqsanger.gz

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ChIP-seq analysis reveals the direct targets of GltR in the P. aeruginosa genome

Project description:we performed ChIP-seq assays to identify in vivo targets of GltR. The plasmid mini-gltR-flag-lacz was constructed and the resultant plasmid was fused to P. aeruginosa strain PAO1, yielding PAO1/mini-gltR-flag-lacz. We investigated GltR-binding to the chromosome of PAO1 during growth with glucose by ChIP-Seq. Sequence reads obtained from three independent ChIP-Seq experiments using anti-flag antibody and mapped to the P. aeruginosa PAO1 genome.Using the MACS software,we identified 55 enriched loci (q-value < 0.05) harboring GltR-binding peaks, that were enriched > 3-fold, but were absent in control sample conducted without anti-flag antibody.

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