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Maintenance of cell fate by the Polycomb Group gene Sex combs extra enables a partial epithelial mesenchymal transition in Drosophila (Pol2 dataset)

ABSTRACT: Maintenance of cell fate by the Polycomb Group gene Sex combs extra enables a partial epithelial mesenchymal transition in Drosophila (Pol2 dataset)

PROVIDER: PRJNA644504 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12161875.fastq.gz	Fastqsanger.gz
	SRR12161876.fastq.gz	Fastqsanger.gz
	SRR12161877.fastq.gz	Fastqsanger.gz
	SRR12161878.fastq.gz	Fastqsanger.gz
	SRR12161879.fastq.gz	Fastqsanger.gz

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Maintenance of cell fate by the Polycomb Group gene Sex combs extra enables a partial epithelial mesenchymal transition in Drosophila (Pol2 dataset)

Project description:Epigenetic silencing by Polycomb group (PcG) complexes can promote epithelial-mesenchymal transition (EMT) and stemness and is associated with malignancy of solid cancers. Here we report a role for Drosophila PcG repression in a partial EMT event that occurs during wing disc eversion, an early event during metamorphosis. In a screen for EMT genes required for eversion we identified the PcG genes Sex combs extra (Sce) and Sex combs midleg (Scm). Depletion of Sce or Scm resulted in internalised wings and thoracic clefts, and loss of Sce inhibited the EMT of the peripodial epithelium and basement membrane breakdown, ex vivo. Targeted DamID (TaDa) using Dam-Pol II showed that Sce knockdown caused a genomic transcriptional response consistent with maintenance of an epithelial fate. Surprisingly only 17 genes were significantly upregulated in Sce-depleted cells, including Abd-B, abd-A, caudal, and nubbin. Each of these loci were enriched for Dam-Pc binding. Of the four genes, only Abd-B was robustly upregulated in cells lacking Sce expression. RNAi knockdown of all four genes could partly suppress the Sce RNAi eversion phenotype, though Abd-B had the strongest effect. Our results suggest that in the absence of continued PcG repression peripodial cells express genes such as Abd-B which regulate epithelial state and thereby disrupt eversion. Our results emphasise the important role that PcG suppression can play in maintaining cell states required for morphogenetic events throughout development and suggest that PcG repression of Hox genes may affect epithelial traits that could contribute to metastasis.

2020-12-31 | GSE153903 | GEO

Maintenance of cell fate by the Polycomb Group gene Sex combs extra enables a partial epithelial mesenchymal transition in Drosophila (Pc dataset)

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Maintenance of cell fate by the Polycomb Group gene Sex combs extra enables a partial epithelial mesenchymal transition in Drosophila (Pc dataset)

Project description:Maintenance of cell fate by the Polycomb Group gene Sex combs extra enables a partial epithelial mesenchymal transition in Drosophila (Pc dataset)

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Maintenance of cell fate by the Polycomb Group gene Sex combs extra enables a partial epithelial mesenchymal transition in Drosophila

Project description:Maintenance of cell fate by the Polycomb Group gene Sex combs extra enables a partial epithelial mesenchymal transition in Drosophila

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Chryseobacterium sp. POL2

Project description:Chryseobacterium sp. POL2 Genome sequencing

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Shewanella sp. POL2

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Maintenance of Cell Fate by the Polycomb Group Gene Sex Combs Extra Enables a Partial Epithelial Mesenchymal Transition in <i>Drosophila</i>.

Project description:Epigenetic silencing by Polycomb group (PcG) complexes can promote epithelial-mesenchymal transition (EMT) and stemness and is associated with malignancy of solid cancers. Here we report a role for Drosophila PcG repression in a partial EMT event that occurs during wing disc eversion, an early event during metamorphosis. In a screen for genes required for eversion we identified the PcG genes S ex combs extra (Sce) and S ex combs midleg ( Scm ) Depletion of Sce or Scm resulted in internalized wings and thoracic clefts, and loss of Sce inhibited the EMT of the peripodial epithelium and basement membrane breakdown, ex vivo. Targeted DamID (TaDa) using Dam-Pol II showed that Sce knockdown caused a genomic transcriptional response consistent with a shift toward a more stable epithelial fate. Surprisingly only 17 genes were significantly upregulated in Sce-depleted cells, including Abd-B, abd-A, caudal, and nub bin Each of these loci were enriched for Dam-Pc binding. Of the four genes, only Abd-B was robustly upregulated in cells lacking Sce expression. RNAi knockdown of all four genes could partly suppress the Sce RNAi eversion phenotype, though Abd-B had the strongest effect. Our results suggest that in the absence of continued PcG repression peripodial cells express genes such as Abd-B, which promote epithelial state and thereby disrupt eversion. Our results emphasize the important role that PcG suppression can play in maintaining cell states required for morphogenetic events throughout development and suggest that PcG repression of Hox genes may affect epithelial traits that could contribute to metastasis.

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Targeted protein degradation reveals direct roles of SPT6 in POL2 elongation and termination

Project description:SPT6 is both, an important histone chaperone and POL2 elongation factor but its primary role on transcription in mammalian cells remains open, as no acute depletion system is available. We used targeted protein degradation to rapidly deplete SPT6 and analyzed defects in POL2 behavior by a multi-omics approach and estimated POL2 processivity, elongation rates and termination and compared it to gene transcription. Our data indicate that SPT6 is a crucial factor for POL2 elongation. Unexpectedly, SPT6 has also a vital role in POL2 termination, as acute depletion induces POL2 read through at most protein coding genes. In contrast, acute depletion did not induce spurious intragenic initiation, while this behavior can be induced by long-term depletion of SPT6 and can therefore be attributed to its function as a histone chaperone. In conclusion, targeted protein degradation of SPT6 allows the kinetic discrimination of its function as a histone chaperone and POL2 elongation factor.

2021-06-23 | GSE162264 | GEO

Drosophila esc mutants show Hox-derepression along the AP axis in CNS

Project description:Mutating esc (extra sex-combs), maternal and zygotic, leads to loss of H3K27me3 and affects Hox-patterning along the AP-axis in neurogenesis

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Regulatory architecture of housekeeping genes is driven by promoter assemblies [Pol2]

Project description:Pol2 ChIA-PET in control and Ronin KO ES cells.

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