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Bulk mRNA sequencing of wildtype (WT) and mutator mouse embryonic fibroblasts (MEFs)

ABSTRACT: Bulk mRNA sequencing of wildtype (WT) and mutator mouse embryonic fibroblasts (MEFs)

PROVIDER: PRJNA644646 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12169034_1.fastq.gz	Fastqsanger.gz
	SRR12169034_2.fastq.gz	Fastqsanger.gz
	SRR12169035_1.fastq.gz	Fastqsanger.gz
	SRR12169035_2.fastq.gz	Fastqsanger.gz
	SRR12169036_1.fastq.gz	Fastqsanger.gz

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Bulk mRNA sequencing of wildtype (WT) and mutator mouse embryonic fibroblasts (MEFs)

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Project description:ING1b and GADD45a are nuclear proteins involved in the regulation of cell growth, apoptosis and DNA repair. We previously found that ING1b is essential to target GADD45a-mediated active DNA-demethylation via TET1 to specific loci. In order to study the physiological impact of ING1-GADD45a on DNA methylation in base-resolution genome-wide, we compared the methylation levels of wildtype mouse embryonic fibroblasts (MEFs) with Ing1/Gadd45a double-knockout MEFs via whole genome bisulfite sequencing.

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