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Transcriptomic analyses in brains of exposed F0 zebrafish and their unexposed F1 and F2 offspring after early-life exposure to permethrin 10 microg/L

ABSTRACT: Transcriptomic analyses in brains of exposed F0 zebrafish and their unexposed F1 and F2 offspring after early-life exposure to permethrin 10 microg/L

PROVIDER: PRJNA644883 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12173019.fastq.gz	Fastqsanger.gz
	SRR12173020.fastq.gz	Fastqsanger.gz
	SRR12173021.fastq.gz	Fastqsanger.gz
	SRR12173022.fastq.gz	Fastqsanger.gz
	SRR12173023.fastq.gz	Fastqsanger.gz

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Transcriptomic analyses in brains of exposed F0 zebrafish and their unexposed F1 and F2 offspring after early-life exposure to permethrin 10 microg/L

Project description:In the present study, zebrafish were exposed to permethrin during early-life, and F1 and F2 generations were bred unexposed. Permethrin exposed F0 fish showed a hypoactive phenotype at adulthood, whereas males from the F1 and F2 generations showed a decrease in anxiety-like behavior. Thus, we performed transcriptomic changes to identify underlying molecular mechanisms. They showed limited overlap between sex and generations. In F0, genes and pathways related to glutamatergic synapse activity (Gene Ontology and Reactome databases) were significantly enriched and may explain the behavioral effects. In F1 and F2 generations, mechanisms are less clear as limited changes which could correlate with behavior were observed in the F1 generation.

2021-07-01 | GSE154020 | GEO

Epigenomic (RRBS) analyses in brains of exposed F0 zebrafish and their unexposed F1 and F2 offspring after early-life exposure to permethrin 10 microg/L

Project description:In the present study, zebrafish were exposed to permethrin during early-life, and F1 and F2 generations were bred unexposed. Permethrin exposed F0 fish showed a hypoactive phenotype at adulthood, whereas males from the F1 and F2 generations showed a decrease in anxiety-like behavior. We performed transcriptomic analyses on whole brains (GSE154020), and here, we further performed RRBS analyses to identify whether there was any stable change in DNA methylation that could be linked to the effects observed at other levels of organization. Due to technical issues during sequencing, we had to perform a correction on the raw data (CombaT) in order to remove a batch effect (flow-cell). We made sure that this did not create any false-positive differentially methylated region. Processed files available are the results from methyl calling before batch correction(with a min coverage >= 5 per C). A matrix of corrected data is available as supplementary file.

2021-07-01 | GSE154206 | GEO

Transcriptomic analyses in brains of exposed F0 zebrafish and their unexposed F2 offspring after exposure to a mixture of PCBs and PBDEs

Project description:Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants still present in aquatic environments despite their total or partial ban. Previously, we observed that an environmentally realistic mixture of these compounds affects energy balance, growth, and reproduction in exposed zebrafish (F0), and behavior in their unexposed offspring (F1-F4). In the present work, we performed lipidomic and transcriptomic analyses on brains of zebrafish (F0-F2) from exposed and control lineages to identify molecular changes that could explain the observed phenotypes. The use of both technologies highlighted that F0 zebrafish displayed impaired mitochondrial function and lipid metabolism dysregulation (depletion in triacylglycerols and phospholipids) which can explain disruption of energy homeostasis. A subset of the dysregulated biological pathways (Gene Ontology and Reactome databases) related to energetic metabolism and neurotransmission were inherited in F2. In addition, there were increasing effects on epigenetic pathways from the F0 to the F2 generation. Altogether, we show that the effects of an environmental exposure to PCBs and PBDEs on energetic metabolism as well as neurotransmission extend over 2 generations of zebrafish, possibly due to transgenerational epigenetic inheritance.

2020-10-01 | GSE146175 | GEO

Epigenomic (RRBS) analyses in brains of exposed F0 zebrafish and their unexposed F1 and F2 offspring after early-life exposure to permethrin 10 microg/L

Project description:Epigenomic (RRBS) analyses in brains of exposed F0 zebrafish and their unexposed F1 and F2 offspring after early-life exposure to permethrin 10 microg/L

| PRJNA645421 | ENA

Transcriptomic analyses in brains of exposed F0 zebrafish and their unexposed F2 offspring after exposure to a mixture of PCBs and PBDEs

Project description:Transcriptomic analyses in brains of exposed F0 zebrafish and their unexposed F2 offspring after exposure to a mixture of PCBs and PBDEs

| PRJNA609652 | ENA

Cryptococcus neoformans var. grubii F0 and F2

Project description:Cryptococcus neoformans var. grubii F0 and F2 Genome sequencing

| PRJNA189304 | ENA

Epigenome-Wide Association Study for Pesticide (Permethrin and DEET) Induced DNA Methylation Epimutation Biomarkers for Specific Transgenerational Disease

Project description:Permethrin and N,N-diethyl-meta-toluamide (DEET) are the pesticides and insect repellent most commonly used by humans. These pesticides have been shown to promote the epigenetic transgenerational inheritance of disease in rats. The current study was designed as an epigenome-wide association study (EWAS) to identify potential sperm differential DNA methylation regions (DMRs) for specific transgenerational disease. Outbred Sprague Dawley gestating female rats (F0) were exposed to the pesticide combination including Permethrin and DEET. Their great-grand offspring (F3) were only transgenerationally exposed to pesticides. The transgenerational adult male rat sperm were collected from individuals with single and multiple diseases and compared to non-diseased animals to identify DMRs associated with transgenerational disease transmission. The exposure of gestating female rats to a permethrin and DEET pesticide combination promoted testis disease, prostate disease, kidney disease, and the presence of multiple disease in the subsequent great-grand offspring F3 generation. Interestingly, the majority of the disease specific sperm DMR associated genes were previously found to be linked to relevant disease specific genes.

2020-11-12 | GSE158254 | GEO

mRNA expression in white adipose tissue of adult F2 female offspring from F0-fathers fed a chow or high-fat diet

Project description:The purpose of this study was to investigate whether paternal high-fat diet (HFD) transgenerationally remodels the epigenome of spermatozoa to alter metabolism in the F1 and F2 generation offspring White adipose tissue mRNA expression profiling of F2-female offspring from F0-founders fed either a chow or a chronic HFD challenged. Adult females were challenged or not a high-fat diet for 12 weeks. White adipose tissue was dissected at an endpoint experiment. Rats were subjected to 4 hours fasting prior to anesthesia with pentobarbital and tissue collection.

2017-01-01 | GSE65182 | GEO

mRNA expression in Extensor Digitorum longus (EDL) muscle of adult F2 female offspring from F0-fathers fed a chow or high-fat diet

Project description:The purpose of this study was to investigate whether grandpaternal high-fat diet (HFD) transgenerationally remodels the transcriptome of skeletal muscle EDL muscle mRNA expression profiling of F2-female offspring from F0-founders fed either a chow or a chronic HFD challenged. Adult females were challenge or not a high-fat diet for 12 weeks. EDL muscle was dissected at an endpoint experiment. Rats were subjected to 4 hours fasting prior to anesthesia with pentobarbital and tissue collection.

2017-08-15 | GSE95490 | GEO

Pesticide and Insect Repellent Mixture (Permethrin and DEET) Induces Epigenetic Transgenerational Inheritance of Disease and Sperm Epimutations

Project description:Environmental compounds are known to promote epigenetic transgenerational inheritance of disease. The current study was designed to determine if a “pesticide mixture” (pesticide permethrin and insect repellent N,N-Diethyl-meta-toluamide, DEET) promotes epigenetic transgenerational inheritance of disease and associated DNA methylation epimutations in sperm. Gestating F0 generation female rats were exposed during fetal gonadal sex determination and the incidence of disease evaluated in F1 and F3 generations. There were significant increases in the incidence of total diseases in animals from pesticide lineage F1 and F3 generation animals. Pubertal abnormalities, testis disease, and ovarian disease (primordial follicle loss and polycystic ovarian disease) were increased in F3 generation animals. Analysis of the pesticide lineage F3 generation sperm epigenome identified 363 differential DNA methylation regions (DMR) termed epimutations. Observations demonstrate that a pesticide mixture (permethrin and DEET) can promote epigenetic transgenerational inheritance of adult onset disease and potential sperm epigenetic biomarkers for ancestral environmental exposures.

2014-07-22 | GSE59554 | GEO

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