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SARS-CoV-2 infection in the lungs of human ACE2 transgenic mice causes severe inflammation and immune cell infiltration, and compromised respiratory function

ABSTRACT: SARS-CoV-2 infection in the lungs of human ACE2 transgenic mice causes severe inflammation and immune cell infiltration, and compromised respiratory function

PROVIDER: PRJNA645133 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12185412_1.fastq.gz	Fastqsanger.gz
	SRR12185412_2.fastq.gz	Fastqsanger.gz
	SRR12185413_1.fastq.gz	Fastqsanger.gz
	SRR12185413_2.fastq.gz	Fastqsanger.gz
	SRR12185414_1.fastq.gz	Fastqsanger.gz

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SARS-CoV-2 infection in the lungs of human ACE2 transgenic mice causes severe inflammation and immune cell infiltration, and compromised respiratory function

Project description:We evaluate heterozygous transgenic mice expressing human ACE2 receptor driven by the epithelial cell promoter cytokeratin-18 promoter (K18-hACE2) as a model of SARS-CoV-2 infection. Intranasal inoculation of K18-hACE2 mice with SARS-CoV-2 results in high levels of infection in the lung parenchyma with spread to other organs.

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Type II interferon-dependent antibacterial defense in the lungs is compromised in diabetes

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Project description:BMPR2 mutation causes pulmonary arterial hypertension (PAH); ACE2 treatment can resolve established BMPR2-mediated PAH. The purpose of this study was to uncover the molecular mechanism behind this. Four groups: +/- ACE2 and +/- BMPR2 transgene, two arrays each, each array a pool of three animals.

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Project description:In vitro, ACE2 translocates to the nucleus to induce SARS-CoV-2 replication. Here, using digital spatial profiling of lung tissues from SARS-CoV-2-infected golden Syrian hamsters, we show that a specific and selective peptide inhibitor of nuclear ACE2 (NACE2i) inhibits viral replication two days after SARS-CoV-2 infection. NACE2i also prevents inflammation and macrophage infiltration, and increases NK cell infiltration in bronchioles. NACE2i treatment restores host translation in infected hamster bronchiolar cells.

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2022-12-01 | E-MTAB-11973 | biostudies-arrayexpress

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